• Immunohistochemistry analysis of APCS in paraffin-embedded mouse liver using APCS Rabbit polyclonal antibody (STJ22636) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of APCS in paraffin-embedded rat spleen using APCS Rabbit polyclonal antibody (STJ22636) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using APCS Rabbit polyclonal antibody (STJ22636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Mouse kidney, using APCS Rabbit polyclonal antibody (STJ22636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-APCS antibody (1-223) (STJ22636)

SKU:
STJ22636

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APCS (1-223) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APCS
Gene ID: 325
Uniprot ID: SAMP_HUMAN
Immunogen Region: 1-223
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-223 of human APCS (NP_001630.1).
Immunogen Sequence: MNKPLLWISVLTSLLEAFAH TDLSGKVFVFPRESVTDHVN LITPLEKPLQNFTLCFRAYS DLSRAYSLFSYNTQGRDNEL LVYKERVGEYSLYIGRHKVT SKVIEKFPAPVHICVSWESS SGIAEFWINGTPLVKKGLRQ GYFVEAQPKIVLGQEQDSYG GKFDRSQSFVGEIGDLYMWD SVLPPENILSAYQGTPLPAN ILDWQALNYEIRGYVIIKPL VWV
Tissue Specificity Found in serum and urine.
Post Translational Modifications N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected.
Function Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
Protein Name Serum Amyloid P-Component
Sap
9.5s Alpha-1-Glycoprotein Cleaved Into - Serum Amyloid P-Component(1-203
Database Links Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Serum Amyloid P-Component antibody
Anti-Sap antibody
Anti-9.5s Alpha-1-Glycoprotein Cleaved Into - Serum Amyloid P-Component(1-203 antibody
Anti-APCS antibody
Anti-PTX2 antibody

Information sourced from Uniprot.org

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