Anti-AP3B1 antibody (895-1094) (STJ29099)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AP3B1 (895-1094) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AP3B1 |
| Gene ID | 8546 |
| Uniprot ID | AP3B1_HUMAN |
| Immunogen Region | 895-1094 |
| Immunogen Sequence | FGDKMVSIQITLNNTTDRKI ENIHIGEKKLPIGMKMHVFN PIDSLEPEGSITVSMGIDFC DSTQTASFQLCTKDDCFNVN IQPPVGELLLPVAMSEKDFK KEQGVLTGMNETSAVIIAAP QNFTPSVIFQKVVNVANVGA VPSGQDNIHRFAAKTVHSGS LMLVTVELKEGSTAQLIINT EKTVIGSVLLRELKPVLSQG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 895-1094 of human AP3B1 (NP_003655.3). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Phosphorylated on serine residues. Pyrophosphorylation by 5-diphosphoinositol pentakisphosphate (5-IP7) impairs interaction with KIF3A. Serine pyrophosphorylation is achieved by Mg(2+)-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue. |
| Function | Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. |
| Protein Name | Ap-3 Complex Subunit Beta-1Adaptor Protein Complex Ap-3 Subunit Beta-1Adaptor-Related Protein Complex 3 Subunit Beta-1Beta-3a-AdaptinClathrin Assembly Protein Complex 3 Beta-1 Large Chain |
| Database Links | Reactome: R-HSA-432722Reactome: R-HSA-6802952 |
| Cellular Localisation | Cytoplasmic VesicleClathrin-Coated Vesicle MembranePeripheral Membrane ProteinCytoplasmic SideGolgi ApparatusComponent Of The Coat Surrounding The Cytoplasmic Face Of Coated Vesicles Located At The Golgi Complex |
| Alternative Antibody Names | Anti-Ap-3 Complex Subunit Beta-1 antibodyAnti-Adaptor Protein Complex Ap-3 Subunit Beta-1 antibodyAnti-Adaptor-Related Protein Complex 3 Subunit Beta-1 antibodyAnti-Beta-3a-Adaptin antibodyAnti-Clathrin Assembly Protein Complex 3 Beta-1 Large Chain antibodyAnti-AP3B1 antibodyAnti-ADTB3A antibody |
Information sourced from Uniprot.org