• Immunofluorescence analysis of PC-12 cells using AOC1 Polyclonal Antibody (STJ28005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using AOC1 Polyclonal Antibody (STJ28005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using AOC1 Polyclonal Antibody (STJ28005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using AOC1 antibody (STJ28005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AOC1 antibody (20-300) (STJ28005)

SKU:
STJ28005

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AOC1 (20-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AOC1
Gene ID: 26
Uniprot ID: AOC1_HUMAN
Immunogen Region: 20-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human AOC1 (NP_001082.2).
Immunogen Sequence: EPSPGTLPRKAGVFSDLSNQ ELKAVHSFLWSKKELRLQPS STTTMAKNTVFLIEMLLPKK YHVLRFLDKGERHPVREARA VIFFGDQEHPNVTEFAVGPL PGPCYMRALSPRPGYQSSWA SRPISTAEYALLYHTLQEAT KPLHQFFLNTTGFSFQDCHD RCLAFTDVAPRGVASGQRRS WLIIQRYVEGYFLHPTGLEL LVDHGSTDAGHWAVEQVWYN GKFYGSPEELARKYADGEV
Tissue Specificity Placenta and kidney.
Post Translational Modifications Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
Function Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function.
Protein Name Amiloride-Sensitive Amine Oxidase Copper-Containing
Dao
Diamine Oxidase
Amiloride-Binding Protein 1
Amine Oxidase Copper Domain-Containing Protein 1
Histaminase
Kidney Amine Oxidase
Kao
Database Links Reactome: R-HSA-211945
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Amiloride-Sensitive Amine Oxidase Copper-Containing antibody
Anti-Dao antibody
Anti-Diamine Oxidase antibody
Anti-Amiloride-Binding Protein 1 antibody
Anti-Amine Oxidase Copper Domain-Containing Protein 1 antibody
Anti-Histaminase antibody
Anti-Kidney Amine Oxidase antibody
Anti-Kao antibody
Anti-AOC1 antibody
Anti-ABP1 antibody
Anti-DAO1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance