• Western blot analysis of A-549, using ANGPTL4 antibody (STJ22612) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of Rat lung, using ANGPTL4 antibody (STJ22612) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ANGPTL4 rabbit polyclonal antibody (STJ22612) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using ANGPTL4 rabbit polyclonal antibody (STJ22612) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ANGPTL4 antibody (26-170) (STJ22612)

SKU:
STJ22612

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANGPTL4 (26-170) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANGPTL4
Gene ID: 51129
Uniprot ID: ANGL4_HUMAN
Immunogen Region: 26-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-170 of human ANGPTL4 (NP_647475.1).
Immunogen Sequence: GPVQSKSPRFASWDEMNVLA HGLLQLGQGLREHAERTRSQ LSALERRLSACGSACQGTEG STDLPLAPESRVDPEVLHSL QTQLKAQNSRIQQLFHKVAQ QQRHLEKQHLRIQHLQSQFG LLDHKHLDHEVAKPARRKRL PEMAQ
Tissue Specificity Detected in blood plasma (at protein level). Detected in liver. Detected in white fat tissue and placenta. Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
Post Translational Modifications N-glycosylated. ANGPTL4 N-terminal chain: Forms disulfide-linked dimers and tetramers. Cleaved into a smaller N-terminal chain and a larger chain that contains the fibrinogen C-terminal domain.both cleaved and uncleaved forms are detected in the extracellular space. The cleaved form is not present within the cell.
Function Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis. ANGPTL4 N-terminal chain: Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
Protein Name Angiopoietin-Related Protein 4
Angiopoietin-Like Protein 4
Hepatic Fibrinogen/Angiopoietin-Related Protein
Hfarp Cleaved Into - Angptl4 N-Terminal Chain - Angptl4 C-Terminal Chain
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-381340
Reactome: R-HSA-8963889
Reactome: R-HSA-9762292
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
The Unprocessed Form Interacts With The Extracellular Matrix
This May Constitute A Dynamic Reservoir
A Regulatory Mechanism Of The Bioavailability Of Angptl4 (Probable)
Alternative Antibody Names Anti-Angiopoietin-Related Protein 4 antibody
Anti-Angiopoietin-Like Protein 4 antibody
Anti-Hepatic Fibrinogen/Angiopoietin-Related Protein antibody
Anti-Hfarp Cleaved Into - Angptl4 N-Terminal Chain - Angptl4 C-Terminal Chain antibody
Anti-ANGPTL4 antibody
Anti-ARP4 antibody
Anti-HFARP antibody
Anti-PGAR antibody
Anti-PP1158 antibody
Anti-PSEC0166 antibody
Anti-UNQ171 antibody
Anti-PRO197 antibody

Information sourced from Uniprot.org

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