• Western blot analysis of extracts of various cell lines, using AMH antibody (STJ11100954) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using AMH Rabbit polyclonal antibody (STJ11100954) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using AMH antibody (STJ11100954) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using AMH antibody (STJ11100954) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded mouse testis using AMH rabbit polyclonal antibody (STJ11100954) at dilution of 1:25 (40x lens).
  • Western blot analysis of extracts of various cell lines, using AMH antibody (STJ11100954) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AMH antibody (360-560) (STJ11100954)

SKU:
STJ11100954

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AMH (360-560) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AMH
Gene ID: 268
Uniprot ID: MIS_HUMAN
Immunogen Region: 360-560
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human AMH (NP_000470.3).
Immunogen Sequence: PAPLHDPTSAPWATALARRV AAELQAAAAELRSLPGLPPA TAPLLARLLALCPGGPGGLG DPLRALLLLKALQGLRVEWR GRDPRGPGRAQRSAGATAAD GPCALRELSVDLRAERSVLI PETYQANNCQGVCGWPQSDR NPRYGNHVVLLLKMQVRGAA LARPPCCVPTAYAGKLLISL SEERISAHHVPNMVATECGC R
Tissue Specificity In ovaries, AMH is detected in granulosa cells of early growing follicles.
Post Translational Modifications Preproprotein is proteolytically processed to generate N- and C-terminal cleavage products that homodimerize and associate to form a biologically active non-covalent complex. Binding of the non-covalent complex to AMHR2 induces dissociation of the pro-region from the mature C-terminal dimer. The N-terminal portion of the protein, despite having no intrinsic activity, has the role of amplifying the activity of the C-terminus.
Function Plays an important role in several reproductive functions. Induces Muellerian duct regression during male fetal sexual differentiation. Also plays a role in Leydig cell differentiation and function. In female acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles. AMH signals by binding to a specific type-II receptor, AMHR2, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A), and recruiting SMAD proteins that are translocated to the nucleus to regulate target gene expression.
Protein Name Muellerian-Inhibiting Factor
Anti-Muellerian Hormone
Amh
Muellerian-Inhibiting Substance
Mis
Database Links Reactome: R-HSA-201451
Reactome: R-HSA-9690406
Cellular Localisation Secreted
Alternative Antibody Names Anti-Muellerian-Inhibiting Factor antibody
Anti-Anti-Muellerian Hormone antibody
Anti-Amh antibody
Anti-Muellerian-Inhibiting Substance antibody
Anti-Mis antibody
Anti-AMH antibody
Anti-MIF antibody

Information sourced from Uniprot.org

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