Anti-ALKBH8 antibody (1-230) (STJ29222)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ALKBH8 (1-230) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:2000-1:10000IHC-P:1:100-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALKBH8 |
| Gene ID | 91801 |
| Uniprot ID | ALKB8_HUMAN |
| Immunogen Region | 1-230 |
| Immunogen Sequence | MDSNHQSNYKLSKTEKKFLR KQIKAKHTLLRHEGIETVSY ATQSLVVANGGLGNGVSRNQ LLPVLEKCGLVDALLMPPNK PYSFARYRTTEESKRAYVTL NGKEVVDDLGQKITLYLNFV EKVQWKELRPQALPPGLMVV EEIISSEEEKMLLESVDWTE DTDNQNSQKSLKHRRVKHFG YEFHYENNNVDKDKPLSGGL PDICESFLEKWLRKGYIKHK PDQMTINQYE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2). |
Additional Info
| Tissue Specificity | Widely expressed, with highest expression in spleen, followed by pancreas and lung. |
| Function | Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine.has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. |
| Protein Name | TrnaCarboxymethyluridine(34-5-O-Methyltransferase Alkbh8Alkylated Dna Repair Protein Alkb Homolog 8Alpha-Ketoglutarate-Dependent Dioxygenase Alkbh8S-Adenosyl-L-Methionine-Dependent Trna Methyltransferase Alkbh8 |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | CytoplasmNucleusPredominantly Cytoplasmic |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Carboxymethyluridine(34-5-O-Methyltransferase Alkbh8 antibodyAnti-Alkylated Dna Repair Protein Alkb Homolog 8 antibodyAnti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkbh8 antibodyAnti-S-Adenosyl-L-Methionine-Dependent Trna Methyltransferase Alkbh8 antibodyAnti-ALKBH8 antibodyAnti-ABH8 antibody |
Information sourced from Uniprot.org