![Western blot analysis of lysates from wild type (WT) and ALDH9A1 knockout (KO) 293T cells, using [KO Validated] ALDH9A1 Rabbit pAb (STJ110185) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88504/404498/STJ110185_1__28553.1744890176.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ALDH9A1 knockout (KO) 293T cells, using [KO Validated] ALDH9A1 Rabbit pAb (STJ110185) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and ALDH9A1 knockout (KO) 293T cells, using [KO Validated] ALDH9A1 Rabbit pAb (STJ110185) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/88504/404498/STJ110185_1__28553.1744890176.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ALDH9A1 knockout (KO) 293T cells, using [KO Validated] ALDH9A1 Rabbit pAb (STJ110185) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
Anti-ALDH9A1 antibody (1-180) (STJ110185)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ALDH9A1 (1-180) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALDH9A1 |
| Gene ID | 223 |
| Uniprot ID | AL9A1_HUMAN |
| Immunogen Region | 1-180 |
| Immunogen Sequence | MFLRAGLAALSPLLRSLRPS PVAAMSTGTFVVSQPLNYRG GARVEPADASGTEKAFEPAT GRVIATFTCSGEKEVNLAVQ NAKAAFKIWSQKSGMERCRI LLEAARIIREREDEIATMEC INNGKSIFEARLDIDISWQC LEYYAGLAASMAGEHIQLPG GSFGYTRREPLGVCVGIGAW |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH9A1 (NP_000687.3). |
Additional Info
| Tissue Specificity | Detected in brain (at protein level). High expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain. Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole. |
| Function | Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency (in vitro). Can catalyze the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, but with low efficiency. Catalyzes the oxidation of aldehydes arising from biogenic amines and polyamines. |
| Protein Name | 4-Trimethylaminobutyraldehyde DehydrogenaseTmaba-DhTmabaldhAldehyde Dehydrogenase E3 IsozymeAldehyde Dehydrogenase Family 9 Member A1Formaldehyde DehydrogenaseGamma-Aminobutyraldehyde DehydrogenaseR-Aminobutyraldehyde Dehydrogenase Cleaved Into - 4-Trimethylaminobutyraldehyde Dehydrogenase - N-Terminally Processed |
| Database Links | Reactome: R-HSA-71262 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-4-Trimethylaminobutyraldehyde Dehydrogenase antibodyAnti-Tmaba-Dh antibodyAnti-Tmabaldh antibodyAnti-Aldehyde Dehydrogenase E3 Isozyme antibodyAnti-Aldehyde Dehydrogenase Family 9 Member A1 antibodyAnti-Formaldehyde Dehydrogenase antibodyAnti-Gamma-Aminobutyraldehyde Dehydrogenase antibodyAnti-R-Aminobutyraldehyde Dehydrogenase Cleaved Into - 4-Trimethylaminobutyraldehyde Dehydrogenase - N-Terminally Processed antibodyAnti-ALDH9A1 antibodyAnti-ALDH4 antibodyAnti-ALDH7 antibodyAnti-ALDH9 antibody |
Information sourced from Uniprot.org