| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ALAS2 (50-320) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALAS2 |
| Gene ID: |
212 |
| Uniprot ID: |
HEM0_HUMAN |
| Immunogen Region: |
50-320 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human ALAS2 (NP_001033057.1). |
| Immunogen Sequence: |
VKTHQFLFGIGRCPILATQG PNCSQIHLKATKAGGDSPSW AKGHCPFMLSELQDGKSKIV QKAAPEVQEDVKAFKTGNYV FSYDQFFRDKIMEKKQDHTY RVFKTVNRWADAYPFAQHFS EASVASKDVSVWCSNDYLGM SRHPQVLQATQETLQRHGAG AGGTRNISGTSKFHVELEQE LAELHQKDSALLFSSCFVAN DSTLFTLAKILPGCEIYSDA GNHASMIQGIRNSGAAKFV |
| Tissue Specificity | Isoform 1: Erythroid-specific. Isoform 2: Erythroid-specific. Isoform 3: Erythroid-specific. Isoform 4: Erythroid-specific. |
| Function | Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. Contributes significantly to heme formation during erythropoiesis. Isoform 3: Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. Catalytic activity is 75-85% of isoform 1 activity. Isoform 4: Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. Catalytic activity is 65-75% of isoform 1 activity. |
| Protein Name | 5-Aminolevulinate Synthase - Erythroid-Specific - MitochondrialAlas-E5-Aminolevulinic Acid Synthase 2Delta-Ala Synthase 2Delta-Aminolevulinate Synthase 2 |
| Database Links | Reactome: R-HSA-189451 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinLocalizes To The Matrix Side Of The Mitochondrion Inner MembraneIsoform 4: Mitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-5-Aminolevulinate Synthase - Erythroid-Specific - Mitochondrial antibodyAnti-Alas-E antibodyAnti-5-Aminolevulinic Acid Synthase 2 antibodyAnti-Delta-Ala Synthase 2 antibodyAnti-Delta-Aminolevulinate Synthase 2 antibodyAnti-ALAS2 antibodyAnti-ALASE antibodyAnti-ASB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of PC-12 cells using [KO Validated] KEAP1 Rabbit polyclonal antibody (STJ11104499) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177143/368561/STJ11104499_1__57159.1713134043.jpg?c=1 "Immunofluorescence analysis of PC-12 cells using [KO Validated] KEAP1 Rabbit polyclonal antibody (STJ11104499) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
