| Short Description : | Rabbit polyclonal ALAS1 (1-250) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ALAS1 |
| Gene ID: | 211 |
| Uniprot ID: | HEM1_HUMAN |
| Immunogen Region: | 1-250 |
| Immunogen Sequence: | MESVVRRCPFLSRVPQAFLQ KAGKSLLFYAQNCPKMMEVG AKPAPRALSTAAVHYQQIKE TPPASEKDKTAKAKVQQTPD GSQQSPDGTQLPSGHPLPAT SQGTASKCPFLAAQMNQRGS SVFCKASLELQEDVQEMNAV RKEVAETSAGPSVVSVKTDG GDPSGLLKNFQDIMQKQRPE RVSHLLQDNLPKSVSTFQYD RFFEKKIDEKKNDHTYRVFK TVNRRAHIFPMADDYSDSL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALAS1 (NP_954635.1). |
| Post Translational Modifications | In normoxia, is hydroxylated at Pro-576, promoting interaction with VHL, initiating ubiquitination and subsequent degradation via the proteasome. Ubiquitinated.in normoxia following hydroxylation and interaction with VHL, leading to its subsequent degradation via the proteasome. |
| Function | Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. |
| Protein Name | 5-Aminolevulinate Synthase - Non-Specific - MitochondrialAlas-H5-Aminolevulinic Acid Synthase 1Delta-Ala Synthase 1Delta-Aminolevulinate Synthase 1 |
| Database Links | Reactome: R-HSA-189451Reactome: R-HSA-1989781Reactome: R-HSA-2151201Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinLocalizes To The Matrix Side Of The Mitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-5-Aminolevulinate Synthase - Non-Specific - Mitochondrial antibodyAnti-Alas-H antibodyAnti-5-Aminolevulinic Acid Synthase 1 antibodyAnti-Delta-Ala Synthase 1 antibodyAnti-Delta-Aminolevulinate Synthase 1 antibodyAnti-ALAS1 antibodyAnti-ALAS3 antibodyAnti-ALASH antibodyAnti-OK antibodyAnti-SW-cl.121 antibody |
Information sourced from Uniprot.org
