Anti-AKR1C2 antibody (1-323) (STJ116408)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AKR1C2 (1-323) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AKR1C2 |
| Gene ID | 1646 |
| Uniprot ID | AK1C2_HUMAN |
| Immunogen Region | 1-323 |
| Immunogen Sequence | MDSKYQCVKLNDGHFMPVLG FGTYAPAEVPKSKALEAVKL AIEAGFHHIDSAHVYNNEEQ VGLAIRSKIADGSVKREDIF YTSKLWSNSHRPELVRPALE RSLKNLQLDYVDLYLIHFPV SVKPGEEVIPKDENGKILFD TVDLCATWEAMEKCKDAGLA KSIGVSNFNHRLLEMILNKP GLKYKPVCNQVECHPYFNQR KLLDFCKSKDIVLVAYSALG SHREEPWVDPNSPVLLEDP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C2 (NP_995317.1). |
Additional Info
| Tissue Specificity | Expressed in fetal testes. Expressed in fetal and adult adrenal glands. |
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids. |
| Protein Name | Aldo-Keto Reductase Family 1 Member C2 3-Alpha-Hsd3Chlordecone Reductase Homolog HakrdDihydrodiol Dehydrogenase 2Dd-2Dd2Dihydrodiol Dehydrogenase/Bile Acid-Binding ProteinDd/BabpType Iii 3-Alpha-Hydroxysteroid Dehydrogenase |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member C2 antibodyAnti-3-Alpha-Hsd3 antibodyAnti-Chlordecone Reductase Homolog Hakrd antibodyAnti-Dihydrodiol Dehydrogenase 2 antibodyAnti-Dd-2 antibodyAnti-Dd2 antibodyAnti-Dihydrodiol Dehydrogenase/Bile Acid-Binding Protein antibodyAnti-Dd/Babp antibodyAnti-Type Iii 3-Alpha-Hydroxysteroid Dehydrogenase antibodyAnti-AKR1C2 antibodyAnti-DDH2 antibody |
Information sourced from Uniprot.org