• Immunofluorescence analysis of U-2 OS cells using AKR1C2 Polyclonal Antibody (STJ116408) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using AKR1C2 Polyclonal Antibody (STJ116408) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using AKR1C2 Rabbit polyclonal antibody (STJ116408) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human Colon cancer using AKR1C2 Rabbit polyclonal antibody (STJ116408) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using AKR1C2 Rabbit polyclonal antibody (STJ116408) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using AKR1C2 Rabbit polyclonal antibody (STJ116408) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using AKR1C2 antibody (STJ116408) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AKR1C2 antibody (1-323) (STJ116408)

SKU:
STJ116408

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AKR1C2 (1-323) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKR1C2
Gene ID: 1646
Uniprot ID: AK1C2_HUMAN
Immunogen Region: 1-323
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C2 (NP_995317.1).
Immunogen Sequence: MDSKYQCVKLNDGHFMPVLG FGTYAPAEVPKSKALEAVKL AIEAGFHHIDSAHVYNNEEQ VGLAIRSKIADGSVKREDIF YTSKLWSNSHRPELVRPALE RSLKNLQLDYVDLYLIHFPV SVKPGEEVIPKDENGKILFD TVDLCATWEAMEKCKDAGLA KSIGVSNFNHRLLEMILNKP GLKYKPVCNQVECHPYFNQR KLLDFCKSKDIVLVAYSALG SHREEPWVDPNSPVLLEDP
Tissue Specificity Expressed in fetal testes. Expressed in fetal and adult adrenal glands.
Function Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids.
Protein Name Aldo-Keto Reductase Family 1 Member C2
3-Alpha-Hsd3
Chlordecone Reductase Homolog Hakrd
Dihydrodiol Dehydrogenase 2
Dd-2
Dd2
Dihydrodiol Dehydrogenase/Bile Acid-Binding Protein
Dd/Babp
Type Iii 3-Alpha-Hydroxysteroid Dehydrogenase
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-193807
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member C2 antibody
Anti-3-Alpha-Hsd3 antibody
Anti-Chlordecone Reductase Homolog Hakrd antibody
Anti-Dihydrodiol Dehydrogenase 2 antibody
Anti-Dd-2 antibody
Anti-Dd2 antibody
Anti-Dihydrodiol Dehydrogenase/Bile Acid-Binding Protein antibody
Anti-Dd/Babp antibody
Anti-Type Iii 3-Alpha-Hydroxysteroid Dehydrogenase antibody
Anti-AKR1C2 antibody
Anti-DDH2 antibody

Information sourced from Uniprot.org

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