Anti-AID antibody (N-Term) (STJ71517)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-AID (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 26kDa band observed in Human Tonsil lysates (calculated MW of 24.0kDa according to NP_065712.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | AICDA |
| Gene ID | 57379 |
| Uniprot ID | AICDA_HUMAN |
| Accession Number | NP_065712.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | RRKFLYQFKNVR |
Additional Info
| Post Translational Modifications | Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity. Probably monoubiquitinated on several residues by RNF126. |
| Function | Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. |
| Protein Name | Single-Stranded Dna Cytosine DeaminaseActivation-Induced Cytidine DeaminaseAidCytidine Aminohydrolase |
| Database Links | Reactome: R-HSA-9821002 |
| Cellular Localisation | NucleusCytoplasmCytosolPredominantly CytosolicIn The Presence Of Mcm3ap/GanpRelocalizes To The Nucleus |
| Alternative Antibody Names | Anti-Single-Stranded Dna Cytosine Deaminase antibodyAnti-Activation-Induced Cytidine Deaminase antibodyAnti-Aid antibodyAnti-Cytidine Aminohydrolase antibodyAnti-AICDA antibodyAnti-AID antibody |
Information sourced from Uniprot.org