• Western blot analysis of extracts of various cell lines, using AGFG1 antibody (STJ115461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-AGFG1 antibody (1-280) (STJ115461)

SKU:
STJ115461

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AGFG1 (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGFG1
Gene ID: 3267
Uniprot ID: AGFG1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2).
Immunogen Sequence: MAASAKRKQEEKHLKMLRDM TGLPHNRKCFDCDQRGPTYV NMTVGSFVCTSCSGSLRGLN PPHRVKSISMTTFTQQEIEF LQKHGNEVCKQIWLGLFDDR SSAIPDFRDPQKVKEFLQEK YEKKRWYVPPEQAKVVASVH ASISGSSASSTSSTPEVKPL KSLLGDSAPTLHLNKGTPSQ SPVVGRSQGQQQEKKQFDLL SDLGSDIFAAPAPQSTATAN FANFAHFNSHAAQNSANAD
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications O-glycosylated.
Function Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.
Protein Name Arf-Gap Domain And Fg Repeat-Containing Protein 1
Hiv-1 Rev-Binding Protein
Nucleoporin-Like Protein Rip
Rev-Interacting Protein
Rev/Rex Activation Domain-Binding Protein
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Nucleus
Cytoplasmic Vesicle
Alternative Antibody Names Anti-Arf-Gap Domain And Fg Repeat-Containing Protein 1 antibody
Anti-Hiv-1 Rev-Binding Protein antibody
Anti-Nucleoporin-Like Protein Rip antibody
Anti-Rev-Interacting Protein antibody
Anti-Rev/Rex Activation Domain-Binding Protein antibody
Anti-AGFG1 antibody
Anti-HRB antibody
Anti-RAB antibody
Anti-RIP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance