Anti-ADH5 antibody (1-374) (STJ115420)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ADH5 (1-374) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ADH5 |
| Gene ID | 128 |
| Uniprot ID | ADHX_HUMAN |
| Immunogen Region | 1-374 |
| Immunogen Sequence | MANEVIKCKAAVAWEAGKPL SIEEIEVAPPKAHEVRIKII ATAVCHTDAYTLSGADPEGC FPVILGHEGAGIVESVGEGV TKLKAGDTVIPLYIPQCGEC KFCLNPKTNLCQKIRVTQGK GLMPDGTSRFTCKGKTILHY MGTSTFSEYTVVADISVAKI DPLAPLDKVCLLGCGISTGY GAAVNTAKLEPGSVCAVFGL GGVGLAVIMGCKVAGASRII GVDINKDKFARAKEFGATE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5/GSNOR (NP_000662.3). |
Additional Info
| Function | Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation. |
| Protein Name | Alcohol Dehydrogenase Class-3Alcohol Dehydrogenase 5Alcohol Dehydrogenase Class Chi ChainAlcohol Dehydrogenase Class-IiiGlutathione-Dependent Formaldehyde DehydrogenaseFaldhFdhGsh-FdhS-(Hydroxymethylglutathione Dehydrogenase |
| Database Links | Reactome: R-HSA-71384 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Alcohol Dehydrogenase Class-3 antibodyAnti-Alcohol Dehydrogenase 5 antibodyAnti-Alcohol Dehydrogenase Class Chi Chain antibodyAnti-Alcohol Dehydrogenase Class-Iii antibodyAnti-Glutathione-Dependent Formaldehyde Dehydrogenase antibodyAnti-Faldh antibodyAnti-Fdh antibodyAnti-Gsh-Fdh antibodyAnti-S-(Hydroxymethylglutathione Dehydrogenase antibodyAnti-ADH5 antibodyAnti-ADHX antibodyAnti-FDH antibody |
Information sourced from Uniprot.org