• Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Actin, smooth muscle (ABT-SMA) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Rabbit stomachPredicted band size: 42kDaObserved band size: 42kDa
  • Human appendix tissue was stained with anti-SMA (ABT-SMA) antibody.
  • Human cardiac muscle tissue was stained with anti-SMA (ABT-SMA) antibody.
  • Human colon tissue was stained with anti-SMA (ABT-SMA) antibody.
  • Human leiomyoma tissue was stained with anti-SMA (ABT-SMA) antibody.
  • Human skeletal muscle tissue was stained with anti-SMA (ABT-SMA) antibody.
  • Human smooth muscle tissue was stained with anti-SMA (ABT-SMA) antibody.
  • Fluorescence multiplex immunohistochemical analysis of normal human appendix tissue (formalin-fixed paraffin-embedded section). The section was incubated in 3 rounds of staining; in the order of Actin. (STJ196911 1/200 dilution), Beta-catenin. (STJ197008 1/200 dilution), MUM1. (STJ197046 1/200 dilution), each using a separate fluorescent tyramide signal amplification system : Treble-Fluorescence immunohistochemical mouse/rabbit kit (pH9.0)

Anti-Actin, smooth muscle antibody (1-100 aa) [ABT-SMA] (STJ196911)

SKU:
STJ196911

Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Actin, smooth muscle (1-100 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT-SMA
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:300-500
IF 1:50-200
WB 1:200-1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTA2
ACTG2
Gene ID: 59
72
Uniprot ID: ACTA_HUMAN
ACTH_HUMAN
Immunogen Region: 1-100 aa
Specificity: This antibody detects endogenous levels of human Actin, smooth muscle (SMA). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin sectio
Immunogen: Synthesized peptide derived from the human Actin, smooth muscle (SMA) at the amino acid range 1-100
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance