• Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 Rabbit polyclonal antibody (STJ118068) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 Rabbit polyclonal antibody (STJ118068) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Acetyl-Histone H2A-K5 Rabbit polyclonal antibody (STJ118068) at 1:1000 dilution. C2C12 cells and C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Acetyl-Histone H2A-K5 antibody (STJ118068)

SKU:
STJ118068

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2AC4.H2AC8
H2AC11.H2AC13.H2AC15.H2AC16.H
Gene ID: 3012/8335
8329/8330/8332/8336/8969
Uniprot ID: H2A1B_HUMAN
H2A1_HUMAN
Specificity: A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
Immunogen Sequence: GRGKQ
Tissue Specificity
Alternative Antibody Names Anti-Histone H2a Type 1-B/E antibody
Anti-Histone H2a.2 antibody
Anti-Histone H2a/A antibody
Anti-Histone H2a/M antibody
Anti-H2AC4 antibody
Anti-H2AFM antibody
Anti-HIST1H2AB.H2AC8 antibody
Anti-H2AFA antibody
Anti-HIST1H2AE antibody
Anti-Histone H2a Type 1 antibody
Anti-H2a.1 antibody
Anti-Histone H2a/Ptl antibody
Anti-H2AC11 antibody
Anti-H2AFP antibody
Anti-HIST1H2AG.H2AC13 antibody
Anti-H2AFC antibody
Anti-HIST1H2AI.H2AC15 antibody
Anti-H2AFD antibody
Anti-HIST1H2AK.H2AC16 antibody
Anti-H2AFI antibody
Anti-HIST1H2AL.H2AC17 antibody
Anti-H2AFN antibody
Anti-HIST1H2AM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance