• Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (STJ118068) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (STJ118068) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human breast using H2AK5ac antibody (STJ118068) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse liver using H2AK5ac antibody (STJ118068) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (STJ118068) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Anti-Acetyl-Histone H2A-K5 antibody (STJ118068)

SKU:
STJ118068
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal primary antibody against Histone H2A.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:50-1:100
Immunogen: A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
1 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Information sourced from uniprot.org

6 months standard