• Western blot analysis of extracts of various cell lines, using ACAP2 antibody (STJ114021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using ACAP2 rabbit polyclonal antibody (STJ114021) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ACAP2 antibody (50-160) (STJ114021)

SKU:
STJ114021

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACAP2 (50-160) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACAP2
Gene ID: 23527
Uniprot ID: ACAP2_HUMAN
Immunogen Region: 50-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human ACAP2 (NP_036419.3).
Immunogen Sequence: KAFCVANKQFMNGIRDLAQY SSNDAVVETSLTKFSDSLQE MINFHTILFDQTQRSIKAQL QNFVKEDLRKFKDAKKQFEK VSEEKENALVKNAQVQRNKQ HEVEEATNILT
Tissue Specificity Widely expressed. Highest level in lung.
Function GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).
Protein Name Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 2
Centaurin-Beta-2
Cnt-B2
Cellular Localisation Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 2 antibody
Anti-Centaurin-Beta-2 antibody
Anti-Cnt-B2 antibody
Anti-ACAP2 antibody
Anti-CENTB2 antibody
Anti-KIAA0041 antibody

Information sourced from Uniprot.org

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