Western blot analysis of various lysates using ACAP2 Rabbit pAb (STJ114021) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-ACAP2 antibody (50-160) (STJ114021)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ114021
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General Information

Short DescriptionRabbit polyclonal ACAP2 (50-160) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolACAP2
Gene ID23527
Uniprot IDACAP2_HUMAN
Immunogen Region50-160
Immunogen SequenceKAFCVANKQFMNGIRDLAQY SSNDAVVETSLTKFSDSLQE MINFHTILFDQTQRSIKAQL QNFVKEDLRKFKDAKKQFEK VSEEKENALVKNAQVQRNKQ HEVEEATNILT
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human ACAP2 (NP_036419.3).

Additional Info

Tissue Specificity Widely expressed. Highest level in lung.
Function GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6). Doesn't show GAP activity for RAB35.
Protein Name Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 2
Centaurin-Beta-2
Cnt-B2
Database Links
Cellular Localisation Cell Membrane
Endosome Membrane
Peripheral Membrane Protein
Colocalizes With Rab35 At The Membrane Protrusions Of Hek293t Cells
Alternative Antibody Names Anti-Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 2 antibody
Anti-Centaurin-Beta-2 antibody
Anti-Cnt-B2 antibody
Anti-ACAP2 antibody
Anti-CENTB2 antibody
Anti-KIAA0041 antibody

Information sourced from Uniprot.org

Citations

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