• Immunohistochemistry analysis of ABCF1 in paraffin-embedded human colon using ABCF1 Rabbit monoclonal antibody (STJ11103057) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ABCF1 Rabbit monoclonal antibody (STJ11103057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ABCF1 antibody (746-845) [S7MR] (STJ11103057)

SKU:
STJ11103057

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ABCF1 (746-845) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCF1
Gene ID: 23
Uniprot ID: ABCF1_HUMAN
Immunogen Region: 746-845
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 746-845 of human ABCF1 (Q8NE71).
Immunogen Sequence: GQKARVVFAELACREPDVLI LDEPTNNLDIESIDALGEAI NEYKGAVIVVSHDARLITET NCQLWVVEEQSVSQIDGDFE DYKREVLEALGEVMVSRPRE
Tissue Specificity Ubiquitous.
Post Translational Modifications Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.
Function Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Protein Name Atp-Binding Cassette Sub-Family F Member 1
Atp-Binding Cassette 50
Tnf-Alpha-Stimulated Abc Protein
Database Links Reactome: R-HSA-382556
Cellular Localisation Isoform 2: Cytoplasm
Nucleus
Nucleoplasm
Nucleus Envelope
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family F Member 1 antibody
Anti-Atp-Binding Cassette 50 antibody
Anti-Tnf-Alpha-Stimulated Abc Protein antibody
Anti-ABCF1 antibody
Anti-ABC50 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance