• Immunofluorescence analysis of U-2 OS cells using PMP70/PMP70/ABCD3 Rabbit polyclonal antibody (STJ11100138) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PMP70/PMP70/ABCD3 Rabbit polyclonal antibody (STJ11100138) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PMP70/PMP70/ABCD3 Rabbit polyclonal antibody (STJ11100138) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PMP70/ABCD3 in paraffin-embedded mouse testis using PMP70/ABCD3 Rabbit polyclonal antibody (STJ11100138) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PMP70/ABCD3 in paraffin-embedded rat kidney using PMP70/ABCD3 Rabbit polyclonal antibody (STJ11100138) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PMP70/ABCD3 Rabbit polyclonal antibody (STJ11100138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ABCD3 antibody (340-475) (STJ11100138)

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STJ11100138

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCD3 (340-475) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCD3
Gene ID: 5825
Uniprot ID: ABCD3_HUMAN
Immunogen Region: 340-475
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-475 of human PMP70/ABCD3 (NP_002849.1).
Immunogen Sequence: LSHPRHLKSTHSELLEDYYQ SGRMLLRMSQALGRIVLAGR EMTRLAGFTARITELMQVLK DLNHGKYERTMVSQQEKGIE GVQVIPLIPGAGEIIIADNI IKFDHVPLATPNGDVLIRDL NFEVRSGANVLICGPN
Post Translational Modifications Ubiquitinated by PEX2 during pexophagy in response to starvation, leading to its degradation.
Function Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation. Has fatty acyl-CoA thioesterase and ATPase activities. Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes. Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation.
Protein Name Atp-Binding Cassette Sub-Family D Member 3
70 Kda Peroxisomal Membrane Protein
Pmp70
Database Links Reactome: R-HSA-1369062
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9603798
Cellular Localisation Peroxisome Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family D Member 3 antibody
Anti-70 Kda Peroxisomal Membrane Protein antibody
Anti-Pmp70 antibody
Anti-ABCD3 antibody
Anti-PMP70 antibody
Anti-PXMP1 antibody

Information sourced from Uniprot.org

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