• Immunohistochemistry analysis of paraffin-embedded mouse lung using ABCA8A Rabbit polyclonal antibody (STJ11103347) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ABCA8A antibody (STJ11103347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ABCA8A antibody (690-828) (STJ11103347)

SKU:
STJ11103347

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCA8A (690-828) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Abca8a
Gene ID: 217258
Uniprot ID: ABC8A_MOUSE
Immunogen Region: 690-828
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 690-828 of mouse ABCA8A (NP_694785.3).
Immunogen Sequence: LKCAGSSLFLKKKWGIGYHL SLQLSETCVHERITSLVKQH IPDSKLSAESEGKLSYILPL ERTNKFPDLYRDLERSPDLG IENYGVSITTLTEVFLKLEG KSSIDQSDIGMTEDVQAGGA RSPERFAEVEQLVSLLNGR
Tissue Specificity Expressed in lung, heart, liver, skeletal muscle and testis. Highly expressed in the liver, and is also abundant in heart and skeletal muscle. Highly expressed in heart.
Function Mediates cholesterol and taurocholate efflux. Through the interaction with ABCA1 potentiates the cholesterol efflux to lipid-free APOA1, in turn regulates high-density lipoprotein cholesterol levels.
Protein Name Abc-Type Organic Anion Transporter Abca8a
Atp-Binding Cassette Sub-Family A Member 8a
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Predominantly Expressed On The Sinusoidal Plasma Membrane
Alternative Antibody Names Anti-Abc-Type Organic Anion Transporter Abca8a antibody
Anti-Atp-Binding Cassette Sub-Family A Member 8a antibody
Anti-Abca8a antibody

Information sourced from Uniprot.org

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