STJ71461 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-AADACL1 antibody (C-Term) (STJ71461)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71461-100
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General Information

Short DescriptionGoat polyclonal antibody anti-AADACL1 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Mouse/Dog/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:1000.
WB: Preliminary experiments gave an approx 26-28kDa band in A431 cell lysates and in Human Hippocampus lysates after 1ยตg/ml antibody staining. This band was successfully blocked by incubati
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolNCEH1
Gene ID57552
Uniprot IDNCEH1_HUMAN
Accession NumberNP_065843.3; NP_001139748.2; NP_001139749.1
Immunogen RegionC-Term
Immunogen SequenceRTRNSYIKWLDQN

Additional Info

Post Translational Modifications N-glycosylated.
Function Hydrolyzes 2-acetyl monoalkylglycerol ether (1-O-alkyl-2-acetyl-sn-glycerol), the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for the hydrolysis of cholesterol esters (such as cholesteryl (9Z-octadecenoate)) in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.
Protein Name Neutral Cholesterol Ester Hydrolase 1
Nceh
Acetylalkylglycerol Acetylhydrolase
2-Acetyl Mage Hydrolase
Arylacetamide Deacetylase-Like 1
Database Links Reactome: R-HSA-8964038
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Microsome
Alternative Antibody Names Anti-Neutral Cholesterol Ester Hydrolase 1 antibody
Anti-Nceh antibody
Anti-Acetylalkylglycerol Acetylhydrolase antibody
Anti-2-Acetyl Mage Hydrolase antibody
Anti-Arylacetamide Deacetylase-Like 1 antibody
Anti-NCEH1 antibody
Anti-AADACL1 antibody
Anti-KIAA1363 antibody

Information sourced from Uniprot.org

Citations

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