| Short Description : | Rabbit monoclonal A2M (1200-1300) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S0MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:200-1:2000IF/ICC:1:100-1:400ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | A2M |
| Gene ID: | 2 |
| Uniprot ID: | A2MG_HUMAN |
| Immunogen Region: | 1200-1300 |
| Immunogen Sequence: | QAPSAEVEMTSYVLLAYLTA QPAPTSEDLTSATNIVKWIT KQQNAQGGFSSTQDTVVALH ALSKYGAATFTRTGKAAQVT IQSSGTFSSKFQVDNNNRLL L |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human Alpha-2-Macroglobulin (A2M) (P01023). |
| Tissue Specificity | Secreted in plasma. |
| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. |
| Protein Name | Alpha-2-MacroglobulinAlpha-2-MC3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140837Reactome: R-HSA-1474228Reactome: R-HSA-8963896 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Alpha-2-Macroglobulin antibodyAnti-Alpha-2-M antibodyAnti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5 antibodyAnti-A2M antibodyAnti-CPAMD5 antibodyAnti-FWP007 antibody |
Information sourced from Uniprot.org
