Active Mouse CSF-1/M-CSF protein (Recombinant) (C-His) (STJP002734)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenActive Recombinant Mouse CSF-1/M-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu262) of mouse M-CSF/CSF-1 (Accession #NP_031804.3.) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Active Mouse CSF-1/M-CSF-C-His protein was developed in hek293 cells using the region Met1-Glu262. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×106-1.0×107units/m |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Csf1 |
| Gene ID | 12977 |
| Uniprot ID | CSF1_MOUSE |
| Immunogen | Active Recombinant Mouse CSF-1/M-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu262) of mouse M-CSF/CSF-1 (Accession #NP_031804.3.) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Met1-Glu262 |
| Immunogen Sequence | MTARGAAGRCPSSTWLGSRL LLVCLLMSRSIAKEVSEHCS HMIGNGHLKVLQQLIDSQME TSCQIAFEFVDQEQLDDPVC YLKKAFFLVQDIIDETMRFK DNTPNANATERLQELSNNLN SCFTKDYEEQNKACVRTFHE TPLQLLEKIKNFFNETKNLL EKDWNIFTKNCNNSFAKCSS RDVVTKPDCNCLYPKATPSS DPASASPHQPPAPSMAPLAG LAWDDSQRTEGSSLLPSEL |
Additional Info
| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. |
| Protein Name | Macrophage Colony-Stimulating Factor 1Csf-1McsfProteoglycan Macrophage Colony-Stimulating FactorPg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-449836Reactome: -MMU-8957275Reactome: -MMU-9856649 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinProcessed Macrophage Colony-Stimulating Factor 1: SecretedExtracellular Space |
| Alternative Protein Names | Macrophage Colony-Stimulating Factor 1 proteinCsf-1 proteinMcsf proteinProteoglycan Macrophage Colony-Stimulating Factor proteinPg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit proteinCsf1 proteinCsfm protein |
Information sourced from Uniprot.org