WEE2 Positive Control for STJ503524 peptide (STJ505664)
SPECIFICATIONS
ImmunogenPurified WEE2 protein.
General Information
| Short Description | WEE2 Positive Control for STJ503524 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | WB: 1:2, 500-1:5, 000 |
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Wee2 |
| Gene ID | 381759 |
| Uniprot ID | WEE2_MOUSE |
| Immunogen | Purified WEE2 protein. |
| Specificity | This is positive control is recommended for use in combination with WEE2 antibody STJ503524. |
Additional Info
| Post Translational Modifications | Phosphorylated by PKA at Ser-15 in vitro, leading to activate kinase activity. Phosphorylation at Ser-15 by CaMK2, leading to increase its activity and promote metaphase II exit during egg activation. |
| Function | Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation. |
| Peptide Name | Wee1-Like Protein Kinase 2Wee1-Like Protein Kinase 1bWee1b KinaseMwee1b |
| Cellular Localisation | CytoplasmNucleusLocalizes Mainly In The NucleusExported From The Nucleus To The Cytoplasm Before Germinal Vesicle Breakdown (Gvbd)Allowing Meiosis Resumption |
| Alternative Peptide Names | Wee1-Like Protein Kinase 2 proteinWee1-Like Protein Kinase 1b proteinWee1b Kinase proteinMwee1b proteinWee2 proteinWee1b protein |
Information sourced from Uniprot.org