| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
ULK3 Positive Control for STJ503467 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Ulk3 |
| Gene ID: |
691171 |
| Uniprot ID: |
ULK3_RAT |
| Specificity: |
This is positive control is recommended for use in combination with ULK3 antibody STJ503467. |
| Post Translational Modifications | Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU. |
| Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. |
| Peptide Name | Serine/Threonine-Protein Kinase Ulk3Unc-51-Like Kinase 3 |
| Database Links | Reactome: R-RNO-5632684 |
| Cellular Localisation | CytoplasmLocalizes To Pre-Autophagosomal Structure During Cellular Senescence |
| Alternative Peptide Names | Serine/Threonine-Protein Kinase Ulk3 proteinUnc-51-Like Kinase 3 proteinUlk3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
