| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | TSG101 Positive Control for STJ503422 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | CD300LF |
| Gene ID: | 146722 |
| Uniprot ID: | CLM1_HUMAN |
| Specificity: | This is positive control is recommended for use in combination with TSG101 antibody STJ503422. |
| Immunogen: | Purified protein. |
| Tissue Specificity | Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells. |
| Post Translational Modifications | Phosphorylated on tyrosine. |
| Function | Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition.recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands. May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2. Inhibits osteoclast formation. Induces macrophage cell death upon engagement. |
| Peptide Name | Cmrf35-Like Molecule 1 Clm-1 Cd300 Antigen-Like Family Member F Immune Receptor Expressed On Myeloid Cells 1 Irem-1 Immunoglobulin Superfamily Member 13 Igsf13 Nk Inhibitory Receptor Cd Antigen Cd300f |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Peptide Names | Cmrf35-Like Molecule 1 protein Clm-1 protein Cd300 Antigen-Like Family Member F protein Immune Receptor Expressed On Myeloid Cells 1 protein Irem-1 protein Immunoglobulin Superfamily Member 13 protein Igsf13 protein Nk Inhibitory Receptor protein Cd Antigen Cd300f protein CD300LF protein CD300F protein CLM1 protein IGSF13 protein IREM1 protein NKIR protein UNQ3105 protein PRO10111 protein |
Information sourced from Uniprot.org