TNFAIP3 Blocking Peptide for STJ500012 peptide (STJ503615)
General Information
| Short Description | TNFAIP3 Blocking Peptide for STJ500012 is synthetically produced from the 690-790 sequence and is suitable for use in western blot applications. |
| Applications | Immunodepletion/Immunocompetition |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Formulation | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | TNFAIP3 |
| Gene ID | 7128 |
| Uniprot ID | TNAP3_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 690-790 on human Tumor necrosis factor alpha-induced protein 3. |
| Immunogen Region | 690-790 |
| Specificity | This blocking peptide is recommended for use in combination with TNFAIP3 antibody, STJ500012 |
Additional Info
| Post Translational Modifications | Proteolytically cleaved by MALT1 upon TCR stimulation.disrupts NF-kappa-B inhibitory function and results in increased IL-2 production. It is proposed that only a fraction of TNFAIP3 colocalized with TCR and CBM complex is cleaved, leaving the main TNFAIP3 pool intact. |
| Function | Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways.affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG.the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin.polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of pro-inflammatory cytokines and IFN beta in LPS-tolerized macrophages. |
| Peptide Name | Tumor Necrosis Factor Alpha-Induced Protein 3Tnf Alpha-Induced Protein 3Otu Domain-Containing Protein 7cPutative Dna-Binding Protein A20Zinc Finger Protein A20 Cleaved Into - A20p50 - A20p37 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5689896Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmNucleusLysosomeA20p50: Cytoplasm |
| Alternative Peptide Names | Tumor Necrosis Factor Alpha-Induced Protein 3 proteinTnf Alpha-Induced Protein 3 proteinOtu Domain-Containing Protein 7c proteinPutative Dna-Binding Protein A20 proteinZinc Finger Protein A20 Cleaved Into - A20p50 - A20p37 proteinTNFAIP3 proteinOTUD7C protein |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about TNFAIP3 Blocking Peptide for STJ500012 peptide (STJ503615)
STJ503615 is a blocking peptide intended for use in combination with the TNFAIP3 antibody STJ500012. It is used to confirm antibody specificity through immunodepletion or immunocompetition experiments.
This blocking peptide is a synthetic peptide derived from amino acid region 690-790 of human Tumor necrosis factor alpha-induced protein 3 (TNFAIP3, UniProt P21580).
This blocking peptide is supplied in liquid form at 2.5 mg/ml in PBS, with up to 5% DMSO compatible with the formulation. Orders exceeding 1 mg can be provided in lyophilized powder form or in a buffer of choice.
This product is recommended for immunodepletion and immunocompetition applications, typically to verify that the paired antibody signal is specific to TNFAIP3. It is intended for research use only.
Store at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.