SREBP2 Blocking Peptide is synthetically produced from the 640-690 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 640-690 on human Sterol regulatory element binding protein-2.
Immunogen Region
640-690
Additional Info
Tissue Specificity
Ubiquitously expressed in adult and fetal tissues.
Post Translational Modifications
Sterol regulatory element-binding protein 2: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Cleavage and activation is induced by mediated cholesterol efflux. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Processed sterol regulatory element-binding protein 2: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus.
Function
Sterol regulatory element-binding protein 2: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis. Processed sterol regulatory element-binding protein 2: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates transcription of genes related to cholesterol synthesis pathway.
Peptide Name
Sterol Regulatory Element-Binding Protein 2Srebp-2Class D Basic Helix-Loop-Helix Protein 2Bhlhd2Sterol Regulatory Element-Binding Transcription Factor 2 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 2Transcription Factor Srebf2
Sterol Regulatory Element-Binding Protein 2: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCytoplasmic VesicleCopii-Coated Vesicle MembraneAt High Sterol ConcentrationsThe Scap-Srebp Is Retained In The Endoplasmic ReticulumLow Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The GolgiWhere It Is ProcessedProcessed Sterol Regulatory Element-Binding Protein 2: NucleusTransported Into The Nucleus With The Help Of Importin-BetaDimerization Of The Bhlh Domain Is A Prerequisite For Importin Beta-Dependent Nuclear Import
Alternative Peptide Names
Sterol Regulatory Element-Binding Protein 2 proteinSrebp-2 proteinClass D Basic Helix-Loop-Helix Protein 2 proteinBhlhd2 proteinSterol Regulatory Element-Binding Transcription Factor 2 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 2 proteinTranscription Factor Srebf2 proteinSREBF2 proteinBHLHD2 proteinSREBP2 protein
