| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
SREBP1 Blocking Peptide is synthetically produced from the 950-1000 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
SREBF1 |
| Gene ID: |
6720 |
| Uniprot ID: |
SRBP1_HUMAN |
| Immunogen Region: |
950-1000 |
| Immunogen: |
Synthetic peptide taken within amino acid region 950-1000 on human Sterol regulatory element binding transcription factor 1 protein. |
| Tissue Specificity | Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1A: Predominates in hepatoma cell lines. Also expressed in kidney, brain, white fat, and muscle. Isoform SREBP-1C: Predominantly expressed in liver and adipose tissues. Also expressed in kidney, brain, white fat, and muscle. |
| Post Translational Modifications | Sterol regulatory element-binding protein 1: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding. Processed sterol regulatory element-binding protein 1: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus. |
| Function | Sterol regulatory element-binding protein 1: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis. Processed sterol regulatory element-binding protein 1: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation. Isoform SREBP-1A: Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C. Able to stimulate both lipogenic and cholesterogenic gene expression. Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver. Required for innate immune response in macrophages by regulating lipid metabolism. Isoform SREBP-1C: Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A. Primarily controls expression of lipogenic gene. Strongly activates global lipid synthesis in rapidly growing cells. Isoform SREBP-1aDelta: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters. Isoform SREBP-1cDelta: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters. |
| Peptide Name | Sterol Regulatory Element-Binding Protein 1Srebp-1Class D Basic Helix-Loop-Helix Protein 1Bhlhd1Sterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1Transcription Factor Srebf1 |
| Database Links | Reactome: R-HSA-1368082Reactome: R-HSA-1655829Reactome: R-HSA-191273Reactome: R-HSA-1989781Reactome: R-HSA-2426168Reactome: R-HSA-381340 P36956-1Reactome: R-HSA-9029558 P36956-3Reactome: R-HSA-9615017 |
| Cellular Localisation | Sterol Regulatory Element-Binding Protein 1: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCytoplasmic VesicleCopii-Coated Vesicle MembraneAt High Sterol ConcentrationsThe Scap-Srebp Is Retained In The Endoplasmic ReticulumLow Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The GolgiWhere It Is ProcessedProcessed Sterol Regulatory Element-Binding Protein 1: NucleusIsoform Srebp-1adelta: NucleusIsoform Srebp-1cdelta: Nucleus |
| Alternative Peptide Names | Sterol Regulatory Element-Binding Protein 1 proteinSrebp-1 proteinClass D Basic Helix-Loop-Helix Protein 1 proteinBhlhd1 proteinSterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1 proteinTranscription Factor Srebf1 proteinSREBF1 proteinBHLHD1 proteinSREBP1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
