| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Septin 2 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Widely expressed. Up-regulated in liver cancer. |
| Function | Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri. |
| Peptide Name | Septin-2Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 5Nedd-5 |
| Database Links | Reactome: R-HSA-5620912 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleChromosomeCentromereKinetochoreCleavage FurrowMidbodyCell CortexCell ProjectionCilium MembraneCiliumFlagellumIn Metaphase CellsLocalized Within The Microtubule SpindleAt The Metaphase PlateIn Close Apposition To The Kinetochores Of The Congressed ChromosomesIn Cells Undergoing CytokinesisLocalized To The MidbodyThe Ingressing Cleavage FurrowAnd The Central SpindleDuring Bacterial InfectionDisplays A Collar Shape Structure Next To Actin At The Pole Of Invading BacteriaIn Epithelial CellsColocalizes With Polyglutamylated Tubulin Around The Trans-Golgi NetworkAs Well As Juxatnuclear And Proximal Golgi ApparatusLocalizes At The Base Of The Cilia Near The Morphological Distinction Between The Cilia And Plasma MembranesFound In The Sperm Annulus |
| Alternative Peptide Names | Septin-2 proteinNeural Precursor Cell Expressed Developmentally Down-Regulated Protein 5 proteinNedd-5 proteinSEPTIN2 proteinDIFF6 proteinKIAA0158 proteinNEDD5 proteinSEPT2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
