Monoclonal Antibodies

Secondary Antibodies

Monoclonal Secondary Antibodies

Raised against a defined host-species immunoglobulin

Monoclonal secondary antibodies bind the constant region of a primary antibody raised in a specific host species, allowing detection of that primary antibody in western blot, immunohistochemistry, immunofluorescence, ELISA and flow cytometry workflows. As with monoclonal primaries, each monoclonal secondary is produced from a single hybridoma clone, giving it a defined, reproducible epitope.

Browse monoclonal secondary antibodies on this page

How it works

Hybridoma-Based Production

Monoclonal secondary antibodies are generated by immunising a host animal with purified immunoglobulin from a target species, then fusing antibody-producing B cells from that host with myeloma cells to form a hybridoma. The resulting hybridoma is cultured to produce a secondary antibody that recognises a single, defined epitope on the target species' immunoglobulin, most commonly on the Fc region or as a heavy and light chain (H&L) conjugate.

Category benefits

Why use monoclonal secondary antibodies?

✓	A single, defined epitope Because each monoclonal secondary descends from one parent hybridoma clone, it binds the same site on the target immunoglobulin every time, supporting consistent detection across repeat experiments and lots.
✓	Broad conjugation options Our monoclonal secondary range is available across a wide set of labels, including Alexa Fluor dyes, HRP, biotin, FITC, gold and Cy-series fluorophores, so a single clone can be matched to the detection method in use.
✓	Choice of host species Our range includes 357 monoclonal secondary antibodies raised in mouse, rabbit and rat hosts, covering detection of primary antibodies across a range of target species.

Choose by host

Monoclonal Secondary Antibodies by Host

Our monoclonal secondary antibodies are produced in mouse and rabbit hosts. Choose a host below to browse that part of the range.

Hybridoma Mouse Monoclonal Secondary Antibodies Raised in mouse against immunoglobulin from a range of target species, with clones available unconjugated and across HRP, biotin, FITC, gold, AMCA, Cy-series and Alexa Fluor conjugates.

Browse Mouse Secondary Monoclonals →

Hybridoma Rabbit Monoclonal Secondary Antibodies Raised in rabbit against immunoglobulin from a range of target species, offering an alternative host for multiplexed panels where a mouse-derived secondary is not suitable.

Browse Rabbit Secondary Monoclonals →

Where they're used

Typical applications

✓	Western blotting HRP and biotin-conjugated monoclonal secondaries detect the primary antibody bound to a target protein on the membrane, with single-epitope binding supporting consistent band detection across repeat runs.
✓	Immunohistochemistry and immunocytochemistry Monoclonal secondaries visualise a primary antibody bound in tissue sections or fixed cells, supporting expression and localisation studies.
✓	Immunofluorescence and flow cytometry Fluorophore-conjugated monoclonal secondaries, including Alexa Fluor and Cy-series dyes, detect unconjugated primary antibodies bound to surface or intracellular targets.
✓	ELISA and immunoprecipitation Enzyme-conjugated monoclonal secondaries support colorimetric or chemiluminescent detection in ELISA, and can be used to detect a primary antibody-antigen complex pulled down from a lysate.

Need help choosing a secondary antibody?

Contact our technical support team for help matching a secondary antibody to your primary's host species and your detection method. Each product page includes a downloadable datasheet and SDS, with dispatch on overnight priority shipping with gel ice packs.

Looking for more secondary antibodies?

Browse our full range of secondary antibodies by species and conjugation.

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