St John's Laboratory Ltd

Immunohistochemistry analysis of STAT3 in paraffin-embedded human brain tissue using [KO Validated] STAT3 Rabbit monoclonal antibody (STJ11101701) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-STAT3 antibody (600-700) [S1MR] (STJ11101701)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (STJ11102565) at 1:1000 dilution. NIH/3T3 and PC-12 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-STAT3-S727 antibody [S5MR] (STJ11102565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using STAT3 rabbit polyclonal antibody (STJ113737) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-STAT3 antibody (600-700) (STJ113737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Beta-Catenin antibody (STJ113822). Western blot was performed from the immunoprecipitate using Beta-Catenin antibody (STJ113822) at a dilution of 1:1000.

Anti-CTNNB1 antibody (667-781) (STJ113822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using Phospho-Beta-Catenin-S552 Rabbit pAb (STJ29345) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-CTNNB1-S552 antibody (STJ29345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-CTNNB1-Ser37 antibody (3-52 aa) (STJ90207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Catenin-Beta (T41/S45) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CTNNB1-Thr41/S45 antibody (11-60 aa) (STJ90208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from COS7 cells treated with UV 15', using Catenin-beta (Phospho-Tyr489) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Tyr489 antibody (455-504 aa) (STJ90928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot with Anti-CTNNB1 Antibody (STJ500625) with peptide on at 1:500 dilution in buffer.

Anti-CTNNB1 antibody (740-790) (STJ500625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Western blot with Anti-Phospho-STAT3 Antibody (STJ502696) of THP cells. Antibody at 1:500 dilution in buffer.

Anti-Phospho-STAT3 antibody (700-750) (STJ502696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Acetyl-CTNNB1-Lys49 antibody (STJA0005926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-CTNNB1-Tyr-489 antibody (STJA0005306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-CTNNB1-Tyr-654 antibody (STJA0005307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Human CTNNB1 protein (Recombinant) (N-His) (STJP004598)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-Phospho-Beta-Catenin-Tyr-489 antibody (STJA0041156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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WB analysis A431 cells Stat1Stat3and Stat5 detection with and without EGF treatment STJA0041380

Anti-Stat3 antibody (N-Term) [M263] (STJA0041380)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP

Reactivity :

Human/Mouse/Rat

£474.50

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Anti-beta Catenin antibody [100000] (STJA0032617)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Monkey/Mouse/Rat

£224.50 - £308.50

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Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CTNNB1-Ser675 antibody (STJ196478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Catenin-beta (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Ser33 antibody (17-66 aa) (STJ90206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts from NIH-3T3 cells, using Phospho-Catenin-Beta (S552) Polyclonal Antibody.

Anti-Phospho-CTNNB1-Ser552 antibody (STJ91364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Catenin-Beta Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (3-52 aa) (STJ92041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Catenin-Beta 1 Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (626-675 aa) (STJ92042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti CTNNB1 Goat Polyclonal antibody immunohistochemistry mouse STJ140021

Anti-CTNNB1 antibody (731aa C-Term) (STJ140021)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £440.50

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Anti-Phospho-STAT3 antibody (700-750) {Biotin} (STJ502697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£551.50

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beta Catenin Blocking Peptide peptide (STJ503875)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Immunohistochemistry analysis of Beta-Catenin in paraffin-embedded human skin using Beta-Catenin Mouse monoclonal antibody (STJ11103229) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Beta-Catenin antibody (300-400 aa) [S9MM] (STJ11103229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Beta-catenin antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 94 kDa

Anti-CTNNB1 antibody (700-781 aa) [ABT-Catenin] (STJ197008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-Phospho-CTNNB1-Tyr-86 antibody (STJA0005303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of Beta-catenin. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-beta Catenin antibody [SM4939] (STJA0004939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-BC2 Tag antibody (PDRKAAVSHWQQ) [SAA2177] (STJA0022375)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

£172.50 - £240.50

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Anti-STAT3 antibody [SRM15052] (STJ11109782)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£118.50 - £521.50

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Anti-Beta-Catenin antibody [IHC516] (STJA0042093)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

£123.50 - £317.50

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Anti-Catenin beta antibody (C-SNQLAWFDTDL) (STJA0041039)

Clonality :

Polyclonal

Host :

Sheep

Applications :

ICC/WB

Reactivity :

Human/Mouse/Rat

£601.50

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Anti-Phospho-Beta-Catenin-Tyr-333 antibody (STJA0041158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Beta-catenin antibody western blot HCT116 C6 3T3 STJA0033269

Anti-beta Catenin antibody [5D6-D5-E9] (STJA0033269)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-STAT3 Ser727 antibody western blot Jurkat C6 HeLa STJA0034891

Anti-Phospho-STAT3-Ser727 antibody [R05-7B2] (STJA0034891)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from 293T cells, using Phospho-Beta-Catenin-S33/S37 Rabbit mAb (STJ11102571) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CTNNB1-S33/S37 antibody [S1MR] (STJ11102571)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Immunofluorescence analysis of U2OS cells using Beta-Catenin antibody (STJ113475) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CTNNB1 antibody (1-100) (STJ113475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-S727 Rabbit pAb (STJ116371) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-STAT3-S727 antibody (STJ116371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry of rat tissue with Anti-CTNNB1 primary antibody (STJ500624) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CTNNB1 antibody (50-100) (STJ500624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-CTNNB1 antibody (50-100) {FITC} (STJ500627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot with Anti-STAT3 Antibody (STJ503137) on recombinant STAT3 protein. Antibody at 1:1500 dilution in buffer.

Anti-STAT3 antibody (700-750) (STJ503137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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STJ73756 (0. 1µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CTNNB1/catenin beta-1 antibody (Internal) (STJ73756)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Anti-CTNNB1 antibody (N-Term) (STJA0005302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Human Catenin beta-1 protein (Recombinant) (C-His) (STJP001253)

Host :

E.coli

£96.50 - £185.50

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Anti-Recombinant-beta Catenin antibody [SR0543] (STJA0010543)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-CTNNB1 nanobody [SAA1410] (STJN000526)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Catenin beta antibody (C-QSYLDSGIHSGATTTAPSL) (STJA0041038)

Clonality :

Polyclonal

Host :

Sheep

Applications :

ICC/IHC-F/WB

Reactivity :

Human/Mouse/Rat

£601.50

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