SARS-CoV-2 SARS-CoV-2 (SARS-CoV-2 Nucleocapsid Protein) Sandwich ELISA Kit (STJE0006698)

SKU:
STJE0006698

Current Stock:
Applications: ELISA
Reactivity: SARS-CoV-2
Note: FOR RESEARCH USE ONLY (RUO).
Sensitivity: 0.12 ng/mL
Detection Limit: 0.39~25ng/mL
Short Description: This SARS-CoV-2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in sars-cov-2 cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Detection: Colorimetric
Sample Type: human serum, plasma and other biological fluids
Specificity This kit recognizes SARS-CoV-2 N protein in samples. No significant cross-reactivity or interference between SARS-CoV-2 N protein and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 82-115 97
EDTA plasma (n=8) 85-98 87

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.21 3.04 10.90 1.31 3.21 11.08
Standard deviation 0.06 0.13 0.54 0.09 0.16 0.59
CV (%) 4.96 4.28 4.95 6.87 4.98 5.32
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance