| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
RUNX1/AML1 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood. |
| Post Translational Modifications | Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A. Methylated. Phosphorylated in Ser-249 Thr-273 and Ser-276 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation. |
| Function | Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells. Positively regulates the expression of RORC in T-helper 17 cells. Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Isoform AML-1L interferes with the transactivation activity of RUNX1. |
| Peptide Name | Runt-Related Transcription Factor 1Acute Myeloid Leukemia 1 ProteinCore-Binding Factor Subunit Alpha-2Cbf-Alpha-2Oncogene Aml-1Polyomavirus Enhancer-Binding Protein 2 Alpha B SubunitPea2-Alpha BPebp2-Alpha BSl3-3 Enhancer Factor 1 Alpha B SubunitSl3/Akv Core-Binding Factor Alpha B Subunit |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-549127Reactome: R-HSA-8877330Reactome: R-HSA-8931987Reactome: R-HSA-8934593Reactome: R-HSA-8935964Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-8939242Reactome: R-HSA-8939243Reactome: R-HSA-8939245Reactome: R-HSA-8939246Reactome: R-HSA-8939247Reactome: R-HSA-8939256Reactome: R-HSA-8941333Reactome: R-HSA-8951936Reactome: R-HSA-9018519Reactome: R-HSA-9616222Reactome: R-HSA-9692916 |
| Cellular Localisation | Nucleus |
| Alternative Peptide Names | Runt-Related Transcription Factor 1 proteinAcute Myeloid Leukemia 1 Protein proteinCore-Binding Factor Subunit Alpha-2 proteinCbf-Alpha-2 proteinOncogene Aml-1 proteinPolyomavirus Enhancer-Binding Protein 2 Alpha B Subunit proteinPea2-Alpha B proteinPebp2-Alpha B proteinSl3-3 Enhancer Factor 1 Alpha B Subunit proteinSl3/Akv Core-Binding Factor Alpha B Subunit proteinRUNX1 proteinAML1 proteinCBFA2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
