RIP140 Blocking Peptide is synthetically produced from the 740-690 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 740-690 on mouse receptor interacting protein 140.
Immunogen Region
740-690
Additional Info
Tissue Specificity
Expressed in the embryonic placenta. In the adult, expression is strong in the testis and brain. Also expressed at a high level in the white adipose tissue. Expressed constantly but at a weaker level in the adult heart, lung, stomach and kidney. Expressed moderately in the skeletal muscle. Expressed at a low level in the adult spleen, liver and brown adipose tissue. Expressed in the ovary at a high level in granulosa cells and at a lower level in the thecal and interstitial compartments.
Post Translational Modifications
Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH. Acetylation regulates its nuclear translocation and corepressive activity.
Function
Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC. Involved in the regulation of ovarian function. Plays a role in renal development.
Peptide Name
Nuclear Receptor-Interacting Protein 1Nuclear Factor Rip140Receptor-Interacting Protein 140
