RICH2 Blocking Peptide is synthetically produced from the 1-60 aa sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide corresponding to N terminal amino acids 1-60 of Mouse RICH2. Sequence: MKKQFNRMRQ LANQTVGRA E KTEVLSED LL QVEKRLE LVK QVSHST HKKL TACLQ GQQGA
Immunogen Region
1-60 aa
Additional Info
Tissue Specificity
Specifically expressed in brain (at protein level). Detected in olfactory bulb, cortex, hippocampus, diencephalon and cerebellum (at protein level). Expressed in hippocampal neurons (at protein level).
Function
GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1. In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity. Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation. In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation.
Peptide Name
Rho Gtpase-Activating Protein 44Rho-Type Gtpase-Activating Protein Rich2Rhogap Interacting With Cip4 Homologs Protein 2Rich-2
Cell ProjectionDendritic SpineRecycling EndosomePresynapseDendriteIn Ca1 Hippocampal SynapsesDetected At Both Presynaptic And Postsynaptic SitesLocated In Convoluted Dendritic Plasma Membrane Sections Enriched In Polymerized Actin And Myosin (Patches) Along Dendrites Where Often Emerge Filopodia
Alternative Peptide Names
Rho Gtpase-Activating Protein 44 proteinRho-Type Gtpase-Activating Protein Rich2 proteinRhogap Interacting With Cip4 Homologs Protein 2 proteinRich-2 proteinArhgap44 proteinRich2 protein
