Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CRHBP antibody (70-150 aa) (STJ192426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

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Western blot analysis of various lysates using CRHR1 Rabbit pAb (STJ110707) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-CRHR1 antibody (24-120) (STJ110707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of PC12 cells using CRF-RI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CRHR1 antibody (71-120 aa) (STJ96855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CRHR2 (PTR1319) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Neuro-2aPredicted band size: 47kDaObserved band size: 47kDa

Anti-CRHR2 antibody (50-150) [PTR1205] (STJA0006274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CRHR2 antibody (71-120 aa) (STJ92475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using CRK/CRK/CRKII Rabbit mAb (STJ11102497) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CRK antibody (1-100) [S7MR] (STJ11102497)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of extracts of various cell lines, using CRK/CRKII antibody (STJ23224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CRK antibody (1-304) (STJ23224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of various cells using Crk II Polyclonal Antibody

Anti-CRK antibody (187-236 aa) (STJ92477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

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Western blot analysis using Crk II monoclonal antibody against LNCAP, A431, MCF7, HL1080 cell lysate.

Anti-CRK antibody (STJ98480)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Dog/Rabbit

$205.62 - $496.80

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Western blot analysis using Crk II monoclonal antibody against HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CRK antibody [3G11E8] (STJ97969)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

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Western blot detection of CrkII in Lncap, A431, MCF7 and HT1080 cell lysates using CrkII mouse mAb (1:1000 diluted).Predicted band size: 34kDa.Observed band size: 34kDa.

Anti-CRK antibody [3H7-E5-H8] (STJ99163)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using CRKL Antibody (STJ113330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CRKL antibody (1-303) (STJ113330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using Crk-L Polyclonal Antibody

Anti-CRKL antibody (173-222 aa) (STJ92478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

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Western blot analysis of extracts of various cell lines, using CRKL antibody (STJ29807) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CRKL antibody (250-350) (STJ29807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-CRKL antibody (C-Term) (STJA0005356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$440.91

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Western blot analysis using Crk-L monoclonal antibody against human Crk-L (AA: 100-303) recombinant protein.

Anti-CRKL antibody [5E12] (STJ97970)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-CRM1/XPO1 antibody [SRM13068] (STJ11107798)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200

Anti-CSF2 antibody (100-144 aa) (STJ98701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

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Western blot analysis using GM-CSF monoclonal antibody against CSF2-hIgGFc transfected HEK293 cell lysate.

Anti-CSF2 antibody (18-144 aa) [3D3] (STJ98113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$205.62 - $496.80

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Immunofluorescence analysis of U-2 OS cells using CSF2 antibody (STJ27880) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CSF2 antibody (20-36) (STJ27880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-CSF2/GM-CSF antibody [SAA0418] (STJA0027595)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $471.27

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Anti-CSF2/GM-CSF antibody [SAA0418] {APC} (STJA0028984)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $584.43

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Anti-CSF2/GM-CSF antibody [SAA0418] {FITC} (STJA0028018)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $471.27

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Anti-CSF2/GM-CSF antibody [SAA0418] {PerCP} (STJA0029950)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$366.39 - $547.17

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Anti-CSF2/GM-CSF antibody [SAA0418] {PE} (STJA0030916)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $584.43

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Western blot analysis of L929 cells using CD116 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CSF2RA antibody (121-170 aa) (STJ96627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

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Immunofluorescence analysis of HeLa cells using CSF2RA antibody (STJ23238) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CSF2RA antibody (23-150) (STJ23238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of various lysates, using CD116/GM-CSFR Alpha antibody (STJ11104972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CSF2RA antibody (23-320 aa) [S4972RM] (STJ11104972)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of various lysates, using CSF2RB Rabbit polyclonal antibody (STJ112525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CSF2RB antibody (200-443) (STJ112525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of various cells using IL-3R Beta Polyclonal Antibody

Anti-CSF2RB antibody (559-608 aa) (STJ93698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CSK Rabbit monoclonal antibody (STJ11101252) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CSK antibody (1-100) [S2MR] (STJ11101252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using CSK Rabbit polyclonal antibody (STJ23240) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Exposuretime: 90s.

Anti-CSK antibody (1-200) (STJ23240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using CSK rabbit polyclonal antibody (STJ11102784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSK antibody (188-450) (STJ11102784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of Jurkat cells using Csk Polyclonal Antibody

Anti-CSK antibody (31-80 aa) (STJ92497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Csk Polyclonal Antibody diluted at 1：1000

Anti-CSK antibody (330-379 aa) (STJ92496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot detection of CSK in C6 and Jurkat cell lysates using CSK mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.

Anti-CSK antibody [2B4-C6-A10] (STJ99130)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Rat

$205.62 - $496.80

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Western blot analysis using Csk monoclonal antibody against NIH/3T3 (1) , HeLa (2) , COS7 (3) , Jurkat (4) , Raw246.7 (5) , A549 (6) , HL-60 (7) and PC-12 (8) cell lysate.

Anti-CSK antibody [5F3] (STJ97972)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$205.62 - $496.80

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Anti-Csk antibody [CSK-04] (STJ16100298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse

$741.75

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Anti-Csk binding protein antibody [PAG-C1] (STJ16100205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/WB/IHC-P

Reactivity :

Rat/Cow/Human/Mouse

$741.75

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Anti-Csk-binding protein antibody (STJ16101301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IP/WB/IHC-P

Reactivity :

Mouse/Human

$741.75

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Western blot analysis of lysates from MCF7 cells, using CSN2 Rabbit polyclonal antibody (STJ118024) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSN2 antibody (1-100) (STJ118024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Anti-CSN2 antibody (19-69) (STJA0006311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various cells using Casein Kinase I Alpha Polyclonal Antibody

Anti-CSNK1A1 antibody (126-175 aa) (STJ92010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (STJ11102475) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-CSNK1A1 antibody (238-337) [S5MR] (STJ11102475)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunohistochemistry analysis of Casein Kinase 1 alpha (CSNK1A1) in paraffin-embedded human placenta using Casein Kinase 1 alpha (CSNK1A1) Rabbit polyclonal antibody (STJ118665) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CSNK1A1 antibody (250-337) (STJ118665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis using Casein Kinase I Alpha monoclonal antibody against truncated CK1 recombinant protein.

Anti-CSNK1A1 antibody [3C10F7/3C10G5/PMC01/4D12B3/3C10F7C11] (STJ97894)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$205.62 - $496.80

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Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CSNK1E antibody (247-416) (STJ28096)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of LOVO cells using Casein Kinase I Epsilon Polyclonal Antibody

Anti-CSNK1E antibody (276-325 aa) (STJ92016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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