Signal Transduction | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (STJ11105777) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-Phospho-CFL1-Y139 antibody (STJ11105777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CFTR-Ser737 antibody (STJ193221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-Che-1/AATF-Ser477 antibody (STJA0003595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Canine/Mouse/Non-Human Primates/Rat/Sheep/Xenopus

£454.50

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Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit monoclonal antibody (STJ11104149) at1:2000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-CHEK1 antibody [S4149RM] (STJ11104149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit pAb (STJ22071) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CHEK1-S280 antibody (STJ22071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of 293T, using Phospho-Chk1 (S280) Rabbit monoclonal antibody (STJ11105881) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-CHEK1-S280 antibody [S5881RM] (STJ11105881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of 293T, using Phospho-Chk1-S280 Rabbit monoclonal antibody (STJ11105894) at 1:2000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-Phospho-CHEK1-S280 antibody [S5894RM] (STJ11105894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Phospho-CHEK1-S296 antibody (STJ11100972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S296 Rabbit mAb (STJ11102618) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0. 5 uM) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CHEK1-S296 antibody [S8MR] (STJ11102618)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-Chk1-S317 Rabbit pAb (STJ22072). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CHEK1-S317 antibody (STJ22072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit pAb (STJ29344) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-CHEK1-S345 antibody (STJ29344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of 293T cells using Phospho-Chk1 (S280) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser280 antibody (251-300 aa) (STJ90221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser286 antibody (256-305 aa) (STJ90705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HUVEC cells treated with UV 15', using Chk1 (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser296 antibody (266-315 aa) (STJ90849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293T cells using Phospho-Chk1 (S301) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CHEK1-Ser301 antibody (271-320 aa) (STJ90706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T cells using Phospho-Chk1 (S317) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser317 antibody (286-335 aa) (STJ90222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser345 antibody (310-359 aa) (STJ90223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Chimaerin beta 2 antibody (STJ502193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-Chimaerin beta 2 antibody {Biotin} (STJ502194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-Chimaerin beta 2 antibody {FITC} (STJ502195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-Chk1-Ser317 antibody [PT0744R] (STJA0040059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-Chk1-Ser345 antibody [PT0651R] (STJA0039191)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from HeLa cells, using Phospho-IKK Alpha-S180 Rabbit pAb (STJ110846) at 1:1000 dilution. HeLa cells were treated by both TNF-Alpha (20ng/mL) and Calyculin A (100nM) for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CHUK-S180 antibody (STJ110846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-Phospho-CHUK-T23 antibody (STJ11101104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-IKK Alpha (T23) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CHUK-Thr23 antibody (15-64 aa) (STJ90300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, using Phospho-c-Jun-S63 Rabbit pAb (STJ11103730) at 1:1000 dilution. 293T cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-cJun-S63 antibody (STJ11103730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (STJ11103749) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T91 antibody (STJ11103749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (STJ11103751) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T93 antibody (STJ11103751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-Phospho-Cofilin antibody (1-50) (STJ506121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-Cofilin antibody (1-50) {Biotin} (STJ506122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-Cofilin antibody (1-50) {FITC} (STJ506123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Western blot analysis of lysates from Jurkat cells using Phospho-Cortactin-Y466 Rabbit pAb (STJ11107623). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Cortactin-Y466 antibody (STJ11107623)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-Phospho-CREB1-S119 antibody [P5G10] (STJA0019174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat/Bovine

£378.50 - £1,632.50

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Western blot analysis of C6, using Phospho-CREB1-S129 Rabbit polyclonal antibody (STJ11105889) at 1:400 dilution. C6 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-CREB1-S129 antibody (STJ11105889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Anti-Phospho-CREB1-S133 antibody [SAA0304] (STJA0019173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-CREB1-Ser-133 antibody [M415] (STJA0005351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Anti-Phospho-CREB1-Ser-271 antibody (STJA0005352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CREB (Phospho-Ser133) antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-Phospho-CREB1-Ser133 antibody (26-140 aa) [R07-3C4] (STJA0011169)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

£246.50

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Anti-Phospho-CRK-Tyr-221 antibody (STJA0005354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-CRK-Tyr-251 antibody (STJA0005355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Anti-Phospho-CRK-Tyr221 antibody (187-236 aa) (STJ90687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from K-562 cells, using Phospho-CRK/CRKII-Y221 Rabbit pAb (STJ117920) at 1:1000 dilution. K-562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody (STJ117920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb (STJ11102643) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody [S3MR] (STJ11102643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-CRKL-Tyr-207 antibody (STJA0005357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of various cells using Phospho-Crk-L (Y207) Polyclonal Antibody

Anti-Phospho-CRKL-Tyr207 antibody (173-222 aa) (STJ91029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-CRKL-Y207 Rabbit pAb (STJ11107726) at1:500 dilution. NIH/3T3 cells were treated by Î»-PP mixed solution (1ul) at 30C for 30 minutes. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 180s.

Anti-Phospho-CRKL-Y207 antibody (STJ11107726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £642.50

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Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit pAb (STJ117922) at 1:1000 dilution or CRKL antibody (STJ230245). K-562 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CRKL-Y207 antibody (STJ117922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CSF2RB-Tyr593 antibody (559-608 aa) (STJ90799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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