• Western blot analysis of extracts of K-562 cells, using Phospho-CRKL-Y207 antibody (STJ117922) at 1:1000 dilution or CRKL antibody (A11735). K-562 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of Phospho-CRKL-Y207 in paraffin-embedded rat brain using Phospho-CRKL-Y207 Rabbit polyclonal antibody (STJ117922) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Phospho-CRKL-Y207 antibody (STJ117922) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit polyclonal antibody (STJ117922) at 1:1000 dilution or CRKL antibody (A11735). K-562 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-CRKL-Y207 antibody (STJ117922)

SKU:
STJ117922

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-CRKL-Y207 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRKL
Gene ID: 1399
Uniprot ID: CRKL_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1).
Immunogen Sequence: HAYAQ
Function May mediate the transduction of intracellular signals.
Protein Name Crk-Like Protein
Database Links Reactome: R-HSA-170968
Reactome: R-HSA-186763
Reactome: R-HSA-8875555
Reactome: R-HSA-8875656
Reactome: R-HSA-9027284
Reactome: R-HSA-912631
Alternative Antibody Names Anti-Crk-Like Protein antibody
Anti-CRKL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance