Reproduction | Research Area | St John's Laboratory

Western blot analysis of various lysates, using DiMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ11105247) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DiMethyl-H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4C16-K20 antibody (STJ11105247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Anti-DiMethyl-TET2-R1682 antibody (STJ11107448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of extracts of Rat testis, using DMC1 antibody (STJ23393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DMC1 antibody (1-260) (STJ23393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using DMC1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-DMC1 antibody (61-110 aa) (STJ92726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of Rat brain, using EOMES antibody (STJ11100179) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EOMES antibody (463-686) (STJ11100179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using EOMES Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-EOMES antibody (59-108 aa) (STJ92929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of A-549 cells using FKBP6 antibody (STJ29093). Blue: DAPI for nuclear staining.

Anti-FKBP6 antibody (1-327) (STJ29093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-FKBP6 antibody (STJA0009660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$64.80 - $340.20

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2AB1 antibody (49-99 aa) (STJ195305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-H2AC18; H2AC19 antibody (STJA0008100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-H2AC20 antibody (70-120 aa) (STJ197583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-H2AC6 antibody (STJA0008762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$64.80 - $340.20

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Anti-H2AFJ antibody (STJA0004724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$189.68 - $560.93

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Anti-H2AFJ antibody (STJA0004725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$189.68 - $560.93

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Immunofluorescence analysis of U-2 OS cells using Histone H2AFV Rabbit polyclonal antibody (STJ119838) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-H2AFV antibody (1-128) (STJ119838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using Histone H2AX Rabbit polyclonal antibody (STJ113704) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-H2AFX antibody (50-143) (STJ113704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Immunofluorescence analysis of Hela cell. 1, Histone H2A.X Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-H2AX antibody (94-143 aa) (STJ93520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/CoIP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2AZ2 antibody (27-77 aa) (STJ194882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2B1C antibody (STJ193068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Histone H2B using Histone H2B (testis specific) Rabbit polyclonal antibody (STJ11104985) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.

Anti-H2BC1 antibody (STJ11104985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-H2BC11 antibody (58-108 aa) (STJ197695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Histone H2B Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-H2BC12L antibody (10-59 aa) (STJ93522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2BC13 antibody (34-84 aa) (STJ195934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2BC21 antibody (18-68 aa) (STJ194032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-H2BC3 antibody (C-Term) (STJA0005436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

$493.43

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Anti-H2BC3 antibody (N-Term) (STJA0005437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$493.43

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Immunofluorescence analysis of Hela cell. 1, Brp44L Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-H2BC3 antibody [Mix] (STJ97064)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of Hela cell. 1, Histone H3.3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-H3-3A/H3-3B antibody (16-65 aa) (STJ93535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12 antibody (STJA0010013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12 antibody (STJA0010014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12 antibody (STJA0010015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using H3F3B antibody (STJ112258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-H3F3B antibody (1-136) (STJ112258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of MCF7, using Lactic acid-Histone H4-K5 rabbit polyclonal antibody (STJ11101520) at 1:1000 dilution. MCF7 cells were treated by Sodium lactate (100mM) for 24h. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-H4K5la antibody (STJ11101520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ113097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Anti-Histone H2AX antibody (1-143 aa) (STJ11106040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3. 3 rabbit monoclonal antibody (STJ11102163). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Histone H3 antibody (57-136) [S3MR] (STJ11102163)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

$197.78 - $867.38

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Anti-Histone H3 antibody [SM0412] (STJA0010412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Anti-Histone H3.2 antibody (81-131 aa) (STJ194163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-Histone H4 antibody (1-100 aa) (STJ11106815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of NIH/3T3 cells using Histone H4 Rabbit polyclonal antibody (STJ119273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Histone H4 antibody (1-100) (STJ119273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Western blot analysis of various lysates, using Histone H4 Rabbit polyclonal antibody (STJ29382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Histone H4 antibody (1-100) (STJ29382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Immunohistochemistry analysis of Histone H4 in paraffin-embedded mouse kidney using Histone H4 Rabbit monoclonal antibody (STJ11103155) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Histone H4 antibody (24-103) [S5MR] (STJ11103155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

$197.78 - $867.38

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Immunocytochemistry analysis of Histone H4 (green) in Hela using Histone H4 antibody, and DAPI (blue).

Anti-Histone H4 antibody (51-70 aa) [R05-8H9] (STJA0011428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/ChIP

Reactivity :

Human

$332.78

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Immunofluorescence analysis of human-liver tissue. 1, Histone H4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Histone H4 antibody (6-55 aa) (STJ93539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using HSPA2 antibody (STJ110211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HSPA2 antibody (400-639) (STJ110211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-HSPA2 antibody (470-550 aa) (STJ190309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Confocal imaging of paraffin-embedded Rat testis tissue using HSPA2 Rabbit monoclonal antibody (STJ11102321, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HSPA2 antibody (488-637) [S1MR] (STJ11102321)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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