• Immunoprecipitation analysis of 300 Mu g extracts from THP-1 cells using 3 Mu g CD9 Rabbit monoclonal antibody (STJ11101565). Western blot was performed from the immunoprecipitate using CD9 Rabbit monoclonal antibody (STJ11101565) at a dilution of 1:1000.
  • Immunohistochemistry analysis of CD9 in paraffin-embedded human lung cancer using CD9 Rabbit monoclonal antibody (STJ11101565) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CD9 Rabbit monoclonal antibody (STJ11101565) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD9 antibody (129-228) [S5MR] (STJ11101565)

SKU:
STJ11101565

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD9 (129-228) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD9
Gene ID: 928
Uniprot ID: CD9_HUMAN
Immunogen Region: 129-228
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 129-228 of human CD9 (P21926).
Immunogen Sequence: YNKLKTKDEPQRETLKAIHY ALNCCGLAGGVEQFISDICP KKDVLETFTVKSCPDAIKEV FDNKFHIIGAVGIGIAVVMI FGMIFSMILCCAIRRNREMV
Tissue Specificity Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells.
Post Translational Modifications Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. Palmitoylation by ZDHHC2 regulates CD9 expression, association with other tetraspanin family proteins and function in cell adhesion.
Function Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Acts as a receptor for PSG17. Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis.
Protein Name Cd9 Antigen
5h9 Antigen
Cell Growth-Inhibiting Gene 2 Protein
Leukocyte Antigen Mic3
Motility-Related Protein
Mrp-1
Tetraspanin-29
Tspan-29
P24
Cd Antigen Cd9
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1300645
Reactome: R-HSA-5336415
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Membrane
Secreted
Extracellular Exosome
Present At The Cell Surface Of Oocytes
Accumulates In The Adhesion Area Between The Sperm And Egg Following Interaction Between Izumo1 And Its Receptor Izumo1r/Juno
Alternative Antibody Names Anti-Cd9 Antigen antibody
Anti-5h9 Antigen antibody
Anti-Cell Growth-Inhibiting Gene 2 Protein antibody
Anti-Leukocyte Antigen Mic3 antibody
Anti-Motility-Related Protein antibody
Anti-Mrp-1 antibody
Anti-Tetraspanin-29 antibody
Anti-Tspan-29 antibody
Anti-P24 antibody
Anti-Cd Antigen Cd9 antibody
Anti-CD9 antibody
Anti-MIC3 antibody
Anti-TSPAN29 antibody
Anti-GIG2 antibody

Information sourced from Uniprot.org

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