Programmed Cell Death | Research Area | St John's Laboratory

Immunohistochemistry analysis of E-cad. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:5000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-E cadherin antibody [SM4912] (STJA0004912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat/Monkey

£170.50 - £233.50

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Anti-E Cadherin antibody [SM5185] (STJA0005185)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-E-Cadherin antibody (775-874 aa) [SRM] (STJ11107256)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Anti-E-Cadherin antibody [36B5] (STJ16101733)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,020.50

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Anti-E-Cadherin antibody [BS38] (STJ16101355)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Dog/Rat/Mouse/Pig/Sheep/Rabbit

£1,229.50

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Anti-E-Cadherin antibody [EP700Y] (STJ16100361)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£712.50 - £1,233.50

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Anti-E-Cadherin antibody [SMM] (STJ11107419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-E-Cadherin antibody [SRM] (STJ11106906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Human infiltrating breast carcinoma stained with anti-E-cadherin antibody using peroxidase-conjugate and DAB chromogen. Note the membrane staining of carcinoma cells.

Anti-E-cadherin antibody [ZM63] (STJ180309)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £544.50

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Formalin-fixed paraffin-embedded human breast lobular anti-E-Cadherin antibody using peroxidase-conjugate and DAB chromogen. Note the strong membranous staining of ductal cells (top) , whereas tumor cells show weak cytoplasmic staining.

Anti-E-Cadherin antibody [ZR375] (STJ180566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £560.50

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Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-E2F1 antibody (100-170 aa) (STJ98642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-E2F1 antibody (125-165) (STJ500811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£466.50

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Anti-E2F1 antibody (125-165) {Biotin} (STJ500815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-E2F1 antibody (125-165) {FITC} (STJ500816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of C6 cells using E2F1 Polyclonal Antibody (STJ23457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-E2F1 antibody (200-301) (STJ23457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using E2F1 Rabbit polyclonal antibody (STJ11103876) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-E2F1 antibody (301-437) (STJ11103876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using E2F1 Rabbit polyclonal antibody (STJ119131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-E2F1 antibody (301-437) (STJ119131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-E2F1 antibody (338-437) [S9MR] (STJ11101709)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-E2F1 antibody (380-430) (STJ500812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£466.50

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Anti-E2F1 antibody (380-430) {Biotin} (STJ500813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-E2F1 antibody (380-430) {FITC} (STJ500814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot with Anti-E2F1 Antibody (STJ500810) with peptide on at 1:500 dilution in buffer.

Anti-E2F1 antibody (58-93) (STJ500810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£466.50

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Anti-E2F1 antibody (58-93) {Biotin} (STJ500817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-E2F1 antibody (58-93) {FITC} (STJ500818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot detection of E2F-1 in C6, Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-E2F1 antibody [4G8-2C2-C12] (STJ99250)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Rat

£144.00 - £348.00

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Western blot analysis of THP-1, using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ELANE antibody (30-267) (STJ114982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ELANE antibody (STJ192040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Cow

£48.00 - £252.00

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Human colon adenocarcinoma stained with MOC-31 antibody using peroxidase-conjugate and DAB chromogen. Note the membranous staining of tumor cells.

Anti-ESA antibody [MOC‐31] (STJ180098)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £525.50

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Immunofluorescence analysis of NIH/3T3 cells using FADD Rabbit polyclonal antibody (STJ11105573) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FADD antibody (1-100) (STJ11105573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and FADD knockout (KO) HeLa cells, using [KO Validated] FADD Rabbit polyclonal antibody (STJ11100017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FADD antibody (1-208) (STJ11100017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from Rat small intestine, using FADD Rabbit polyclonal antibody (STJ11104486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FADD antibody (1-208) (STJ11104486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Jurkat cells, using FADD Rabbit polyclonal antibody antibody (STJ118887) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FADD antibody (1-208) (STJ118887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g FADD antibody (STJ28382). Western blot was performed from the immunoprecipitate using FADD antibody (STJ28382) at a dilution of 1:1000.

Anti-FADD antibody (1-208) (STJ28382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using FADD Polyclonal Antibody diluted at 1：500

Anti-FADD antibody (159-208 aa) (STJ93021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using FADD antibody (STJ11101587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FADD antibody (20-150) [S7MR] (STJ11101587)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot detection of FADD in A431 and Jurkat cell lysates using FADD mouse mAb (1:1000 diluted).Predicted band size:23KDa.Observed band size:23KDa.

Anti-FADD antibody [7C4-F12-F1] (STJ99305)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using Fas antibody (STJ23628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-FAS antibody (200-335) (STJ23628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human-liver-cancer tissue. 1, FAS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-FAS antibody (257-306 aa) (STJ93041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Dog

£48.00 - £252.00

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Western blot analysis of various cells using CD95 Polyclonal Antibody diluted at 1：500

Anti-FAS antibody (281-330 aa) (STJ92150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat spleen using Fas Rabbit polyclonal antibody (STJ116183) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-FAS antibody (50-150) (STJ116183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Fas in paraffin-embedded rat kidney using Fas Rabbit monoclonal antibody (STJ11101711) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-FAS antibody (50-150) [S1MR] (STJ11101711)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Western blot analysis of extracts of HT-1080 cells, using Fas Rabbit monoclonal antibody antibody (STJ11104597) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FAS antibody (50-150) [S4597RM] (STJ11104597)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of HEB cells using FAS Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-FAS antibody (51-100 aa) (STJ96891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using FAS monoclonal antibody against Jurkat cell lysate.

Anti-FAS antibody (C-Term) (STJ98491)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of FAS (C-terminus) in Jurkat cell lysates using FAS (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.

Anti-FAS antibody (C-Term) [4C3-G7-B7-H5] (STJ99086)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from 1) HepG2, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5G3) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-FASLG antibody (101-150 aa) (STJ93042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Pig

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer, antibody was diluted at 1:100

Anti-FASLG antibody (121-170 aa) (STJ99000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of mouse heart, using FasLG antibody (STJ23630) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-FasLG antibody (154-170) (STJ23630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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