Immune System | Research Area | St John's Laboratory

Western blot analysis of various cells using Ku-86 Polyclonal Antibody

Anti-XRCC5 antibody (441-490 aa) (STJ93869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using Ku80 Rabbit pAb (STJ28425) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-XRCC5 antibody (543-732) (STJ28425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Ku80 antibody (STJ11101409). Western blot was performed from the immunoprecipitate using Ku80 antibody (STJ11101409) at a dilution of 1:1000.

Anti-XRCC5 antibody (633-732) [S9MR] (STJ11101409)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from 1) K562, 2) Jurkat, 3) COS7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-XRCC5 antibody (683-732 aa) (STJ93866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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Anti-XRCC5 antibody (Internal Region) (STJA0005773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Hamster/Human/Mouse/Rat

£455.50

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Anti-XRCC5 antibody (Internal Region) (STJA0005774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Hamster/Human/Mouse/Rat

£455.50

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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-XRCC5 antibody (STJ193184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using Ku-80 monoclonal antibody against HeLa (1) , MCF-7 (2) , A549 (3) and NIH/3T3 (4) cell lysate.

Anti-XRCC5 antibody [5C5] (STJ98211)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot detection of Ku80 in COS7, Jurkat, Hela, MCF7 and A549 cell lysates using Ku80 mouse mAb (1:1000 diluted). Predicted band size:86KDa. Observed band size:86KDa.

Anti-XRCC5 antibody [8H1-C3-G10] (STJ99226)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Monkey

£144.00 - £348.00

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Anti-XRCC5 Ku-86 kd subunit antibody [J15] (STJ16102618)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Monkey

£287.50 - £1,324.50

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Confocal immunofluorescence analysis of U2OS cells using Ku70 Polyclonal Antibody (STJ26134) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-XRCC6 antibody (408-609) (STJ26134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of U-2 OS cells using Ku70 Rabbit monoclonal antibody (STJ11101297, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-XRCC6 antibody (500-609) [S7MR] (STJ11101297)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunoprecipitation analysis of 100 Mu g extracts of SW480 cells using 3 Mu g Ku70 antibody (STJ29469). Western blot was performed from the immunoprecipitate using Ku70 antibody (STJ29469) at a dilution of 1:1000.

Anti-XRCC6 antibody (501-600) (STJ29469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using [KD Validated] Ku70 Rabbit polyclonal antibody (STJ11105550) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-XRCC6 antibody (505-609) (STJ11105550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Ku-70 Polyclonal Antibody

Anti-XRCC6 antibody (560-609 aa) (STJ93863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Ku70 in Hela, A549, COS7 and K562 cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:67KDa.

Anti-XRCC6 antibody [7B1-E12-G4] (STJ99222)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot analysis of extracts of 293T cells, using YB-1/YB-1/YBX1 antibody (STJ11105722). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-YBX1 antibody (1-55) (STJ11105722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of mouse brain using YB-1/YB-1/YBX1 antibody (STJ110017) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-YBX1 antibody (100-200) (STJ110017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using YB-1/YB-1/YBX1 antibody (STJ118142) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-YBX1 antibody (100-200) (STJ118142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of HeLa cells, using YB-1/YBX1 rabbit monoclonal antibody (STJ11101893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YBX1 antibody (225-324) [S3MR] (STJ11101893)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunocytochemistry analysis of YB1 (green) in Hela using YB1 antibody, and DAPI (blue).

Anti-YBX1 antibody (288-301 aa) [R03-6G4] (STJA0012148)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Hamster

£264.50

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Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-YBX1 antibody (68-117 aa) (STJ96290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using YES1 Rabbit polyclonal antibody (STJ29810) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-YES1 antibody (1-250) (STJ29810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis using c-Yes monoclonal antibody against truncated YES1-His recombinant protein (1) and full-length GFP-YES1 (aa1-543) transfected COS7 cell lysate (2).

Anti-YES1 antibody (10-193 aa) [2F3E6] (STJ97980)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot with Anti-YES1 Antibody (STJ503564) on mouse lung. Antibody at 1:250 dilution in buffer.

Anti-YES1 antibody (450-480) (STJ503564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-YES1 antibody (450-480) {Biotin} (STJ503565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-YES1 antibody (450-480) {FITC} (STJ503566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Western blot analysis of various lysates using YPEL5 Rabbit polyclonal antibody (STJ116543) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-YPEL5 antibody (1-121) (STJ116543)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-YPEL5 antibody (STJA0007571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using 14-3-3 alpha/beta Rabbit mAb (STJ11102440) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-YWHAB antibody (147-246) [S0MR] (STJ11102440)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of L929 cells using 14-3-3 alpha/beta Rabbit polyclonal antibody (STJ26141) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-YWHAB antibody (150-246) (STJ26141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using 14-3-3 Beta Polyclonal Antibody

Anti-YWHAB antibody (41-90 aa) (STJ91367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using YWHAZ antibody (STJ115333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-YWHAZ antibody (146-245) (STJ115333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Rat brain using YWHAZ Rabbit polyclonal antibody (STJ29954) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-YWHAZ antibody (146-245) (STJ29954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells, using 14-3-3 zeta/delta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-YWHAZ antibody (196-245 aa) (STJ91373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using 14-3-3 Zeta Polyclonal Antibody diluted at 1：1000

Anti-YWHAZ antibody (24-73 aa) (STJ91372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using ZAP70 antibody (STJ26147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ZAP70 antibody (1-300) (STJ26147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Human tonsil stained with anti-ZAP-70 antibodies using peroxidase-conjugate and DAB chromogen. Note the positive staining of perifollicular B-cells

Anti-ZAP70 antibody (247-382aa) [ZM97] (STJ180332)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £432.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ZAP-70 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ZAP70 antibody (286-335 aa) (STJ96296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ZAP70 antibody (350-410) (STJ506007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-ZAP70 antibody (350-410) {Biotin} (STJ506008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-ZAP70 antibody (350-410) {FITC} (STJ506009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates, using ZAP70 Rabbit mAb (STJ11102484) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-ZAP70 antibody (520-619) [S4MR] (STJ11102484)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Anti-ZAP70 antibody (550-619 aa) [ABT-ZAP70] (STJ196936)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Jurkat whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-ZAP-70 (ABT246) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 70kDaObserved band size: 70kDa

Anti-ZAP70 antibody (550-619 aa) [ABT246] (STJ196737)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis using ZAP-70 monoclonal antibody against Jurkat cell lysate (1).

Anti-ZAP70 antibody [3D2A4] (STJ98456)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of ZAP-70 in CEM and Jurkat cell lysates, negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-ZAP70 antibody [4B2-C1-H3] (STJ99214)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£144.00 - £348.00

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Immunoprecipitation of ZBP1 from 500 µg extracts of 293F cells transfected with ZBP1 was performed using 2 µg of ZBP1 Rabbit mAb (STJ11107360). Rabbit IgG isotype control (STJ11105763) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using ZBP1 Rabbit mAb (STJ11107360) at a dilution of 1 : 3000.

Anti-ZBP1 antibody (1-170 aa) [SRM] (STJ11107360)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/ELISA

Reactivity :

Human

£129.50 - £574.50

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