• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser337) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from MDA-MB-435 cells, using NF-kappaB p105/p50 (Phospho-Ser337) Antibody. The lane on the left is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-NF Kappa B-p105/p50 (S337) Polyclonal Antibody diluted at 1:1000

Anti-Phospho-NFKB1-Ser337 antibody (304-353 aa) (STJ90346)

SKU:
STJ90346

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Nuclear factor NF-kappa-B p105 subunit-Ser337 (304-353 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKB1
Gene ID: 4790
Uniprot ID: NFKB1_HUMAN
Immunogen Region: 304-353 aa
Specificity: Phospho-NF Kappa B-p105/p50 (S337) Polyclonal Antibody detects endogenous levels of NF Kappa B-p105/p50 protein only when phosphorylated at S337.
Immunogen: The antiserum was produced against synthesized peptide derived from the human NF-kappaB p105/p50 around the phosphorylation site of Ser337 at the amino acid range 304-353
Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling.active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. Nuclear factor NF-kappa-B p105 subunit: P105 is the precursor of the active p50 subunit (Nuclear factor NF-kappa-B p50 subunit) of the nuclear factor NF-kappa-B. Acts as a cytoplasmic retention of attached NF-kappa-B proteins by p105. Nuclear factor NF-kappa-B p50 subunit: Constitutes the active form, which associates with RELA/p65 to form the NF-kappa-B p65-p50 complex to form a transcription factor. Together with RELA/p65, binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions.
Protein Name Nuclear Factor Nf-Kappa-B P105 Subunit
Dna-Binding Factor Kbf1
Ebp-1
Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1 Cleaved Into - Nuclear Factor Nf-Kappa-B P50 Subunit
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1810476
Reactome: R-HSA-193692
Reactome: R-HSA-202424
Reactome: R-HSA-209560
Reactome: R-HSA-2559582
Reactome: R-HSA-2871837
Reactome: R-HSA-3134963
Reactome: R-HSA-3214841
Reactome: R-HSA-381340
Reactome: R-HSA-445989
Reactome: R-HSA-448706
Reactome: R-HSA-5603029
Reactome: R-HSA-5607764
Reactome: R-HSA-5621575
Reactome: R-HSA-5660668
Reactome: R-HSA-5684264
Reactome: R-HSA-6798695
Reactome: R-HSA-844456
Reactome: R-HSA-8853884
Reactome: R-HSA-9020702
Reactome: R-HSA-933542
Reactome: R-HSA-9609690
Reactome: R-HSA-9660826
Reactome: R-HSA-9692916
Cellular Localisation Nuclear Factor Nf-Kappa-B P105 Subunit: Cytoplasm
Nuclear Factor Nf-Kappa-B P50 Subunit: Nucleus
Cytoplasm
Association With Nfkbia Inhibitor (I-Kappa-B)
Promotes Its Retention In The Cytoplasm In An Inactive Form
Translocates Into The Nucleus Following Nfkbia Degradation
Alternative Antibody Names Anti-Nuclear Factor Nf-Kappa-B P105 Subunit antibody
Anti-Dna-Binding Factor Kbf1 antibody
Anti-Ebp-1 antibody
Anti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1 Cleaved Into - Nuclear Factor Nf-Kappa-B P50 Subunit antibody
Anti-NFKB1 antibody

Information sourced from Uniprot.org

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