Immune System | Research Area | St John's Laboratory

Western blot analysis of extracts of U-937 cells, using CD18 antibody (STJ11101550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ITGB2 antibody (50-150) [S0MR] (STJ11101550)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of HeLa cells using Integrin Beta 2 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-ITGB2 antibody (621-670 aa) (STJ96826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using CD18/ITGB2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ITGB2 antibody (720-769 aa) (STJ93734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Confocal immunofluorescence analysis of HL60 cells using CD18 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Anti-ITGB2 antibody [10E12] (STJ97909)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of extracts from A549, using Integrin Beta 5/ITGB5 Rabbit monoclonal antibody (STJ11105106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ITGB5 antibody (24-490 aa) [S5106RM] (STJ11105106)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from A549 cells, using ITGB5 Rabbit polyclonal antibody (STJ24261) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB5 antibody (490-720) (STJ24261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Integrin Beta 5 Polyclonal Antibody diluted at 1：1000

Anti-ITGB5 antibody (721-770 aa) (STJ93738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of various lysates using ITGB7 Rabbit polyclonal antibody (STJ27701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB7 antibody (1-140) (STJ27701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of mouse-heart cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-ITGB7 antibody (671-720 aa) (STJ99322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-ITGB7 antibody [SAA1982] (STJA0020410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£212.50 - £1,184.50

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ITK antibody (450-530 aa) (STJ193205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry of mouse Large Intestine with Anti-ITK primary antibody (STJ501509) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 20X.

Anti-ITK antibody (550-600) (STJ501509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-ITK antibody (550-600) {Biotin} (STJ501510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-ITK antibody (550-600) {FITC} (STJ501511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis using Emt monoclonal antibody against Jurkat cell lysate.

Anti-ITK antibody [5G6] (STJ98020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-ITK antibody [R04-8H3] (STJA0036475)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various lysates, using ITK/EMT antibody (STJ11104892) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITK antibody [S4892RM] (STJ11104892)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ITLN1 antibody (110-190 aa) (STJ192342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of mouse colon using ITLN1 Rabbit polyclonal antibody (STJ29314) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ITLN1 antibody (19-313) (STJ29314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of COS7 cells, using InsP3R1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ITPR1 antibody (1566-1615 aa) (STJ93746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293T using IP3R-I antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-ITPR1 antibody (1700-1780 aa) (STJ97616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using IP3R Rabbit pAb (STJ110214) at 1:1000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 90s.

Anti-ITPR1 antibody (1812-1911) (STJ110214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of mouse brain using IP3R Rabbit monoclonal antibody (STJ11102081) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ITPR1 antibody (2200-2300) [S1MR] (STJ11102081)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Anti-ITPR1 antibody (STJ501512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey/Mouse/Rat

£400.50

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Anti-ITPR1 antibody {Biotin} (STJ501513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey/Mouse/Rat

£497.50

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Anti-ITPR1 antibody {FITC} (STJ501514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey/Mouse/Rat

£497.50

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Immunofluorescence analysis of NIH/3T3 cells using ITPR2 Rabbit polyclonal antibody (STJ11103923) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ITPR2 antibody (2415-2480) (STJ11103923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ITPR2 antibody (STJA0004425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£139.50 - £403.50

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Anti-ITPR2 antibody (STJA0004426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

£139.50 - £403.50

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Anti-ITPR2 antibody (STJA0004427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

£139.50 - £403.50

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Western blot analysis of various lysates, using ITPR3 Rabbit monoclonal antibody (STJ11105047) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ITPR3 antibody (1630-1870 aa) [S5047RM] (STJ11105047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of HeLa, using ITPR3 rabbit polyclonal antibody (STJ114939) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITPR3 antibody (1630-1870) (STJ114939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-ITPR3 antibody (STJA0010298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-J1 epitope of COL2A1 antibody [M2139] (STJA0019067)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IHC/WB

Reactivity :

Human/Mouse/Rat/Bovine

£209.50 - £1,333.50

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Anti-JAB1 antibody [R09-4W9] (STJA0036708)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of U2OS cells using JAK1 Rabbit polyclonal antibody (STJ11100279) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-JAK1 antibody (30-300) (STJ11100279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using JAK1 Rabbit monoclonal antibody (STJ11102738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-JAK1 antibody (600-700) [S8MR] (STJ11102738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using JAK1 Rabbit mAb (STJ11107112) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.

Anti-JAK1 antibody (721-820 aa) [SRM5230] (STJ11107112)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Immunofluorescence analysis of U-2 OS cells using JAK1 Rabbit polyclonal antibody (STJ11100912) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-JAK1 antibody (856-875) (STJ11100912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using JAK1 Mouse mAb diluted at 1:200.

Anti-JAK1 antibody (960-1040 aa) [7G6] (STJ97712)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of rat-lung tissue. 1, JAK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-JAK1 antibody (988-1037 aa) (STJ93789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IP/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela Cell, 2) 3T3 Cell, 3) PC12 Cell lysate using JAK1 mouse Monoclonal Antibody diluted at 1:2,000.

Anti-JAK1 antibody [3F12] (STJ196697)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-JAK1 antibody [7B1-B6-G7] (STJA0033203)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of extracts from C6, Ramos, Jurkat and Hela cell lysates using Jak1 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.

Anti-JAK1 antibody [8B8-E7-G3] (STJ99138)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Rat

£149.00 - £360.00

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Anti-JAK1 antibody [8B8-E7-G3] (STJA0033204)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-JAK1 antibody [8D8-2B5-5E3] (STJA0032984)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Anti-JAK1 antibody [PT0658R] (STJA0039224)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-JAK1 antibody [R06-2V6] (STJA0036607)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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