Immune System | Research Area | St John's Laboratory

Immunofluorescence analysis of HepG2 cells using NLRP3 Rabbit polyclonal antibody (STJ27619) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NLRP3 antibody (43-1036) (STJ27619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of K562 cells using Cryopyrin Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NLRP3 antibody (511-560 aa) (STJ96747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded mouse kidney using NLRP3 Rabbit polyclonal antibody (STJ11105476) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NLRP3 antibody (930-1036) (STJ11105476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-NLRP3 antibody [18#] (STJA0019837)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/Neut

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NLRP4 antibody (307-357 aa) (STJ195380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HEPG2 Hela cells using NLRX1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-NLRX1 antibody (581-630 aa) (STJ98633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and NLRX1 knockout (KO) HeLa cells, using [KO Validated] NLRX1 Rabbit polyclonal antibody (STJ11100038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NLRX1 antibody (87-360) (STJ11100038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse kidney using 3 Mu g NLRX1 antibody (STJ116270). Western blot was performed from the immunoprecipitate using NLRX1 (STJ116270) at a dilution of 1:1000.

Anti-NLRX1 antibody (87-975) (STJ116270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using NME2/NM23B antibody (STJ29579) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NME2 antibody (1-152) (STJ29579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various lysates, using NME2/NM23B Rabbit monoclonal antibody (STJ11102312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NME2 antibody (50-152) [S2MR] (STJ11102312)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from K562 cells, using NM23 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-NME2 antibody (91-140) (STJ94512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-nNOS antibody (STJ13100199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using NOD1 antibody (STJ24778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NOD1 antibody (1-270) (STJ24778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NOD1 antibody (290-340 aa) (STJ193684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using NOD2 Rabbit polyclonal antibody (STJ11105602) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOD2 antibody (28-270) (STJ11105602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using NOD2 Rabbit polyclonal antibody (STJ118437) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOD2 antibody (30-1040) (STJ118437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NOD2 antibody (STJA0009810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using NOS1 antibody (STJ114661) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOS1 antibody (1-180) (STJ114661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of rat liver, using NOS1 antibody (STJ24781) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOS1 antibody (1-180) (STJ24781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, NOS1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-NOS1 antibody (818-867) (STJ94533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NOS1 antibody (C-Term) (STJA0005498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-NOS1 antibody (C-Term) [M401] (STJA0005497)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£394.50

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Anti-NOS1 antibody (N-Term) (STJA0005670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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Western blot with Anti-NOS2 Antibody (STJ503603) on 1) RAW cells treated with LPS, 2) RAW cells. Antibody at 1:250 dilution in buffer.

Anti-NOS2 antibody (100-150) (STJ503603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-NOS2 antibody (100-150) {Biotin} (STJ503605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-NOS2 antibody (100-150) {FITC} (STJ503606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western Blot of Anti-NOS2 antibody (STJ503604) with Osteoblast cells (A) and cells treated to induce the INOS protein (B). Cell extracts were made with protease inhibitors and 50-60ug protein was resolved on SDS-PAGE for western blotting. Antibody at 1:500 dilution in buffer.

Anti-NOS2 antibody (1064-1114) (STJ503604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£427.50

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Anti-NOS2 antibody (1064-1114) {Biotin} (STJ503607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-NOS2 antibody (1064-1114) {FITC} (STJ503608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Immunofluorescence analysis of rat-kidney tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-NOS2 antibody (117-166) (STJ94534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from RAW264. 7 cells, using iNOS Rabbit pAb (STJ115966) at 1:700 dilution. Raw264. 7 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-NOS2 antibody (948-1153) (STJ115966)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using iNOS Rabbit polyclonal antibody (STJ11103799) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOS2 antibody (974-1143) (STJ11103799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Anti-NOS2 antibody (C-Term) (STJA0005495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-NOS2 antibody (C-Term) [M398] (STJA0005494)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-NOS2/iNOS antibody [SAA0105] (STJA0026985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-NOS2/iNOS antibody [SAA0105] {APC} (STJA0029394)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-NOS2/iNOS antibody [SAA0105] {FITC} (STJA0028428)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-NOS2/iNOS antibody [SAA0105] {PerCP} (STJA0030360)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£292.50 - £440.50

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Anti-NOS2/iNOS antibody [SAA0105] {PE} (STJA0031326)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ24784) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1000-1203) (STJ24784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ11104517) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1035-1110) (STJ11104517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of eNOS in paraffin-embedded rat spleen tissue using eNOS Rabbit monoclonal antibody (STJ11104147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-NOS3 antibody (1035-1110) [S4147RM] (STJ11104147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, NOS3 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-NOS3 antibody (1145-1194) (STJ94539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HUVEC cells using eNOS Rabbit polyclonal antibody (STJ117269) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (639-738) (STJ117269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NOS3 antibody (C-Term) (STJA0005490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Immunohistochemistry analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues with DAB staining using NOS3 monoclonal antibody.

Anti-NOS3 antibody [6H2] (STJ98282)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from 1) Rat Heart Tissue, 2) huvec, 3) Jurkat cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-NOS3 antibody [Mix] (STJ97066)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Rabbit

£48.00 - £252.00

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Western blot analysis of various lysates, using eNOS antibody (STJ11104808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NOS3 antibody [S4808RM] (STJ11104808)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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