Anti-IL-12A antibody (STJA0037479)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPeptide
General Information
| Short Description | Rabbit monoclonal anti-IL-12A for use in WB, IHC-F, IHC-P and FC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB-1:1000-1:5000IHC-P-1:200-1:1000FC-1:100-1:200 |
| Formulation | Liquid in 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL12A |
| Gene ID | 3592 |
| Uniprot ID | IL12A_HUMAN |
| Immunogen | Peptide |
Additional Info
| Function | Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine. Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites. |
| Protein Name | Interleukin-12 Subunit AlphaIl-12aCytotoxic Lymphocyte Maturation Factor 35 Kda SubunitClmf P35Il-12 Subunit P35Nk Cell Stimulatory Factor Chain 1Nksf1 |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-8984722Reactome: R-HSA-9020591 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-12 Subunit Alpha antibodyAnti-Il-12a antibodyAnti-Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit antibodyAnti-Clmf P35 antibodyAnti-Il-12 Subunit P35 antibodyAnti-Nk Cell Stimulatory Factor Chain 1 antibodyAnti-Nksf1 antibodyAnti-IL12A antibodyAnti-NKSF1 antibody |
Information sourced from Uniprot.org