Immune System | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using LEF1 antibody (STJ114908) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LEF1 antibody (20-132) (STJ114908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using LEF1 Rabbit monoclonal antibody (STJ11102088) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-LEF1 antibody (50-150) [S8MR] (STJ11102088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-LEF1 antibody [PT0601R] (STJA0039283)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-LEF1 antibody [SRM14642] (STJ11109372)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Legumain (LGMN) antibody [SRM14679] (STJ11109409)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Leukocyte Common Antigen antibody [BRA-55] (STJ16100941)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/ELISA/IF/WB

Reactivity :

Human

£408.50

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Western blot with Anti-LGALS3 Antibody (STJ500375) with peptide on at 1:500 dilution in buffer.

Anti-LGALS3 antibody (1-100) (STJ500375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Immunoprecipitation analysis of 300 Mu g extracts of HT-29 cells using 3 Mu g Galectin 3/LGALS3 antibody (STJ11101290). Western blot was performed from the immunoprecipitate using Galectin 3/LGALS3 antibody (STJ11101290) at a dilution of 1:1000.

Anti-LGALS3 antibody (1-100) [S0MR] (STJ11101290)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-LGALS3 antibody (1-100) {Biotin} (STJ500377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-LGALS3 antibody (1-100) {FITC} (STJ500378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LGALS3 antibody (1-250) (STJ115467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, CBP 35 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-LGALS3 antibody (141-190 aa) (STJ92060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of mouse Colon with Anti-LGALS3 primary antibody (STJ500376) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-LGALS3 antibody (150-250) (STJ500376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-LGALS3 antibody (150-250) {Biotin} (STJ500379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-LGALS3 antibody (150-250) {FITC} (STJ500380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Galectin-3 antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 26 (30) kDaObserved band size: 33 kDa

Anti-LGALS3 antibody (200-300 aa) [ABT-GAL3] (STJ197048)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£180.00 - £261.00

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Western blot analysis of 1) MCF7, 2) 3T3 with Galectin-3 Mouse mAb diluted at 1:2, 000.

Anti-LGALS3 antibody [8D7] (STJ97550)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Galectin-9 (PTR1201) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1Predicted band size: 39kDaObserved band size: 37kDaThere is one non-specific band.

Anti-LGALS9 antibody (1-100) [PTR1201] (STJA0006277)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of various lysates using LGALS9 Rabbit pAb (STJ11103608) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-LGALS9 antibody (1-240) (STJ11103608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, Galectin-9 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-LGALS9 antibody (51-100 aa) (STJ93206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-lgE antibody [121] (STJA0027004)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-lgE antibody [121] {APC} (STJA0029728)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-lgE antibody [121] {FITC} (STJA0028762)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-lgE antibody [121] {PerCP} (STJA0030694)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£292.50 - £440.50

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Anti-lgE antibody [121] {PE} (STJA0031660)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-lgM Mu-Chain Specific antibody [HB57] (STJA0027002)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-lgM Mu-Chain Specific antibody [HB57] {APC} (STJA0029730)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-lgM Mu-Chain Specific antibody [HB57] {FITC} (STJA0028764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-lgM Mu-Chain Specific antibody [HB57] {PerCP} (STJA0030696)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£292.50 - £440.50

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Anti-lgM Mu-Chain Specific antibody [HB57] {PE} (STJA0031662)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Immunohistochemistry of mouse tissue with Anti-LGMN primary antibody (STJ501591) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-LGMN antibody (100-150) (STJ501591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-LGMN antibody (100-150) {Biotin} (STJ501592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-LGMN antibody (100-150) {FITC} (STJ501593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of Legumain in Jurkat, Hela lysates using Legumain antibody.

Anti-LGMN antibody (204-311 aa) [R06-7G1] (STJA0011550)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£246.50

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Western blot analysis of extracts of various cell lines, using Legumain (LGMN) (LGMN) Rabbit monoclonal antibody (STJ11102328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LGMN antibody (210-302) [S8MR] (STJ11102328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of mouse kidney, using Legumain (LGMN) antibody (STJ112585) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LGMN antibody (26-288) (STJ112585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LGMN antibody (60-140 aa) (STJ192484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-LGMN/Legumain antibody [SAA1616] (STJA0020044)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of K562 cells using LIF Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-LIF antibody (141-190 aa) (STJ96472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using LIF rabbit polyclonal antibody (STJ29846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LIF antibody (23-202) (STJ29846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry of paraffin embedded human colon cancer using LIF at dilution of 1:1000

Anti-LIF antibody [SM5226] (STJA0005226)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of lysates from mouse skeletal muscle, using LIFR Rabbit polyclonal antibody (STJ114935) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-LIFR antibody (500-700) (STJ114935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Western blot analysis of 293 cells using LIFR Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-LIFR antibody (731-780 aa) (STJ97299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-LIFR/CD118 antibody {ABflo® 594} (STJ11108780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£0.00

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Anti-LIFR/CD118 antibody {ABflo® 647} (STJ11108779)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£0.00

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Anti-LIFR/CD118 antibody {PE} (STJ11108801)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£0.00

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Western blot analysis of various lysates using LILRA1 Rabbit pAb (STJ114005) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-LILRA1 antibody (17-210) (STJ114005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using LIR-6 Polyclonal Antibody

Anti-LILRA1 antibody (53-102 aa) (STJ93934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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