Immune System | Research Area | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using c-Fos Rabbit pAb (STJ119065) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-FOS antibody (1-114) (STJ119065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g c-Fos antibody (STJ23685). Western blot was performed from the immunoprecipitate using c-Fos antibody (STJ23685) at a dilution of 1:1000.

Anti-FOS antibody (1-114) (STJ23685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using c-Fos Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-FOS antibody (1-50 aa) (STJ92249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-FOS antibody (331-380 aa) (STJ92252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with c-Fos Mouse mAb diluted at 1:2, 000.

Anti-FOS antibody [6A3] (STJ97486)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot detection of c-Fos in HCT116, COS7 and C6 cell lysates using c-Fos mouse mAb (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.

Anti-FOS antibody [7D6-A9-D4] (STJ99211)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat/Monkey

£144.00 - £348.00

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Anti-FOS antibody [N486/76] (STJA0019767)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£341.50 - £1,447.50

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Anti-FOS nanobody [SAA1335] (STJN000074)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/IHC

Reactivity :

Human

£378.50 - £1,632.50

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Immunofluorescence analysis of Hela cell. 1, FoxO1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-FOXO1 antibody (223-272 aa) (STJ93123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using FoxO1A Polyclonal Antibody diluted at 1：1000

Anti-FOXO1 antibody (295-344 aa) (STJ93126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Mouse thymus, using FOXO1 Rabbit polyclonal antibody (STJ115801) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-FOXO1 antibody (340-580) (STJ115801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis using FoxO1A monoclonal antibody against A431, HeLa cell lysate.

Anti-FOXO1 antibody (C-Term) (STJ98492)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot detection of FOXO1 (C-terminus) in A431 and Hela cell lysates using FOXO1 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size: 70KDa.

Anti-FOXO1 antibody (C-Term) [3F8-H8-A10] (STJ99101)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using FoxO3A Polyclonal Antibody diluted at 1：1000

Anti-FOXO3 antibody (220-269 aa) (STJ93128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using FOXO3A Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-FOXO3 antibody (281-330 aa) (STJ93127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using FOXO3A Rabbit mAb (STJ11102470) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-FOXO3 antibody (574-673) [S0MR] (STJ11102470)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot with Anti-FOXO3 Antibody (STJ501079) of A. Mouse Ovary, B. Rat Ovary (Rat testis) at 1:500 dilution in buffer.

Anti-FOXO3 antibody (620-670) (STJ501079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-FOXO3 antibody (620-670) {Biotin} (STJ501080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-FOXO3 antibody (620-670) {FITC} (STJ501081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of FOXO3A in Hela, A549 lysates using FOXO3 antibody.

Anti-FOXO3 antibody (660-673 aa) [R03-2H2] (STJA0012161)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£264.50

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-FOXO3 antibody (STJ99616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of rat spleen with Anti-FPR1 primary antibody (STJ501082) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-FPR1 antibody (150-200) (STJ501082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-FPR1 antibody (150-200) {Biotin} (STJ501083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-FPR1 antibody (150-200) {FITC} (STJ501084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Immunofluorescence analysis of A549 cells, using FPR1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-FPR1 antibody (151-200 aa) (STJ93134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of MCF7 cells, using FPR1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-FPR1 antibody (155-204 aa) (STJ93133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-FPR1 antibody (170-220 aa) (STJ13101096)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-FPR1 antibody (2-50 aa) (STJ13101115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Immunohistochemistry analysis of paraffin-embedded human colon using FPR1 Rabbit polyclonal antibody (STJ11103976) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-FPR1 antibody (250-350) (STJ11103976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-FPR1 antibody (300-350 aa) (STJ13101104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Immunofluorescence analysis of PC-12 cells using FPR2 Rabbit polyclonal antibody (STJ11104999) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-FPR2 antibody (1-100 aa) (STJ11104999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-FPR2 antibody (130-210 aa) (STJ192622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse/Swine

£48.00 - £252.00

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Anti-FPR2 antibody (2-50 aa) (STJ13101097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis using Rak monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Rak cDNA (2).

Anti-FRK antibody (2-300 aa) [1A8H2C12] (STJ98353)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using Rak Polyclonal Antibody

Anti-FRK antibody (71-120 aa) (STJ95361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-FRMPD3 antibody (1451-1501 aa) (STJ194478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using Fascin/FSCN1 Rabbit mAb (STJ11102495) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-FSCN1 antibody (249-362) [S5MR] (STJ11102495)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Fascin antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 55 kDa

Anti-FSCN1 antibody (250-350 aa) [ABT-FASN] (STJ196833)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Fascin/Fascin/FSCN1 antibody (STJ115318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-FSCN1 antibody (260-380) (STJ115318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of A549, HuvEc, HT29, K562, L929 cells using Fascin 1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-FSCN1 antibody (261-310 aa) (STJ96577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Fascin/Fascin/FSCN1 Rabbit polyclonal antibody (STJ23712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-FSCN1 antibody (394-493) (STJ23712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-FSCN1 antibody [55K2] (STJA0005418)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of extracts of various cell lines, using Ferritin Heavy Chain antibody (STJ11101689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FTH1 antibody (1-100) [S9MR] (STJ11101689)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of A-549 cells using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FTH1 antibody (1-183) (STJ23718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-FTH1 antibody (120-170 aa) (STJ93057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded human tonsil tissue using Ferritin Heavy Chain Rabbit monoclonal antibody (STJ11104840) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-FTH1 antibody (84-183) [S4840RM] (STJ11104840)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot with Anti-FTH1 Antibody (STJ501085) of purified human Ferritin at 1:250 dilution in buffer.

Anti-FTH1 antibody (Full Length) (STJ501085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-FTH1 antibody (Full Length) {Biotin} (STJ501086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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