• Immunofluorescence analysis of Jurkat cells using CD99 Rabbit polyclonal antibody (STJ23019) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human testis using CD99 Rabbit polyclonal antibody (STJ23019) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human islet using CD99 Rabbit polyclonal antibody (STJ23019) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD99 antibody (STJ23019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CD99 antibody (23-185) (STJ23019)

SKU:
STJ23019

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD99 (23-185) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD99
Gene ID: 4267
Uniprot ID: CD99_HUMAN
Immunogen Region: 23-185
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-185 of human CD99 (NP_002405.1).
Immunogen Sequence: DGGFDLSDALPDNENKKPTA IPKKPSAGDDFDLGDAVVDG ENDDPRPPNPPKPMPNPNPN HPSSSGSFSDADLADGVSGG EGKGGSDGGGSHRKEGEEAD APGVIPGIVGAVVVAVAGAI SSFIAYQKKKLCFKENAEQG EVDMESHRNANAEPAVQRTL LEK
Post Translational Modifications Extensively O-glycosylated.
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.
Protein Name Cd99 Antigen
12e7
E2 Antigen
Protein Mic2
T-Cell Surface Glycoprotein E2
Cd Antigen Cd99
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202733
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cd99 Antigen antibody
Anti-12e7 antibody
Anti-E2 Antigen antibody
Anti-Protein Mic2 antibody
Anti-T-Cell Surface Glycoprotein E2 antibody
Anti-Cd Antigen Cd99 antibody
Anti-CD99 antibody
Anti-MIC2 antibody
Anti-MIC2X antibody
Anti-MIC2Y antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance